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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,672 results for: pCas

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Showing: 261 - 280 of 2672 results

  1. c3GIC9

    Plasmid
    #62191
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. Pyogenes Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62191-plasmid-map-sequence-id-156341

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  2. ABE9

    Plasmid
    #194208
    Purpose
    Mammalian expression of ABE9
    Depositor
    Dali Li
    Publication
    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-8e (N108Q/L145T)-nSpCas9
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194208-plasmid-map-sequence-id-473532

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  3. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62887-plasmid-map-sequence-id-105910

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  4. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62888-plasmid-map-sequence-id-105911

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  5. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Adam Karpf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113194-plasmid-map-sequence-id-216314

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  6. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  7. pX330-EN1201

    Plasmid
    #92144
    Purpose
    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locus
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
    Expression
    Mammalian
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92144-plasmid-map-sequence-id-177211

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  8. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid
    #78311
    Purpose
    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.
    Depositor
    Timo Otonkoski
    Publication
    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78311-plasmid-map-sequence-id-146188

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  11. pX330.puro

    Plasmid
    #110403
    Purpose
    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance
    Depositor
    Sandra Martha Gomes Dias
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110403-plasmid-map-sequence-id-213570

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  13. YE1-BE4max

    Plasmid
    #138155
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138155-plasmid-map-sequence-id-272340

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  15. pCMV-dCas13-M3M14nes

    Plasmid
    #155367
    Purpose
    Targeted m6A RNA methylation in mammalian cells
    Depositor
    David Liu
    Publication
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155367-plasmid-map-sequence-id-305821

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  16. pCMV-Mm.cargo(Cas9)

    Plasmid
    #174865
    Purpose
    Expresses mouse Peg10 cargoRNA encoding SpCas9
    Depositor
    Feng Zhang
    Publication
    Segel et al Science. 2021 Aug 20;373(6557):882-889.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 flanked by MmPeg10 UTRs
    Tags
    HA-SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    174865-plasmid-map-sequence-id-342429

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  18. pSI-Target-AID-NG

    Plasmid
    #119861
    Purpose
    Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.
    Depositor
    Osamu Nureki
    Publication
    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    Expression
    Mammalian
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    119861-plasmid-map-sequence-id-232065

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  19. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139988-plasmid-map-sequence-id-275338

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  20. pAAV-EFS-SpCas9 (AAV8)

    Viral prep
    #104588-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EFS-SpCas9 (#104588). In addition to the viral particles, you will also receive purified pAAV-EFS-SpCas9 plasmid DNA. EFS-driven expression of Myc-tagged SpCas9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ryohei Yasuda
    Publication
    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)
    Promoter
    EFS
    Available since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only
Showing: 261 - 280 of 2672 results