We narrowed to 9,934 results for: plasmid dna

1. pexK4_bow_360

   Plasmid

   #89673

   Purpose

   Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3B of associated article.

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   bow_360
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pexA2_bow_180

   Plasmid

   #89671

   Purpose

   Plasmid for the construction of DNA-protein hybrid nanostructures. Template for the bent two-helix-bundle shown in figure 3A of associated article.

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   bow_180
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti SpBsmBI sgRNA Hygro

   Plasmid

   #62205

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSC27

   Plasmid

   #117478

   Purpose

   mobilizable, SRB-stable, kanamycin-resistant cloning vector

   Depositor

   Judy Wall

   Article

   Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-MCS2

   Plasmid

   #46954

   Purpose

   Cloning plasmid for the generation of Knock-In in human cells using rAAV.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBV-Luc

   Plasmid

   #16539

   Purpose

   luciferase reporter plasmid with very low basal activity

   Depositor

   Bert Vogelstein

   Article

   He et al Cell. 1999 Oct 29. 99(3):335-45.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBIS-GALkFLP(URA)

   Plasmid

   #49460

   Purpose

   overespression of Flp step-arrest mutant in yeast, URA selection

   Depositor

   Marc Gartenberg

   Article

   Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   GAL10

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBIS-GALkFLP(Trp1)

   Plasmid

   #49459

   Purpose

   overespression of Flp step-arrest mutant in yeast, TRP1 selection

   Depositor

   Marc Gartenberg

   Article

   Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   GAL10

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIX_403

   Plasmid

   #41395

   Purpose

   Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET3CTc3A(1-135)

    Plasmid

    #63138

    Purpose

    Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

    Depositor

    Titia Sixma

    Article

    Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

    Insert

    Tc3A
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    1-135 aa bipartite domain

    Promoter

    T7

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET22B_SA_PcrA

    Plasmid

    #102999

    Purpose

    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker

    Depositor

    Martin Webb

    Insert

    SA_PcrA
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    PcrA 199-2423

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET16B.Pfu

    Plasmid

    #12509

    Purpose

    Bacterial expression of Pfu DNA polymerase

    Depositor

    Sung-Hou Kim

    Article

    Goldman et al Acta Crystallogr D Biol Crystallogr. 199

    Insert

    Pfu DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y218H, Q462R, F533L, K559I and V683A (mutations w…

    Promoter

    Available since

    Sept. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAP1836

    Plasmid

    #137087

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAP1837

    Plasmid

    #137086

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAP3041

    Plasmid

    #137083

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    GFP11::3xFlag::tagRFP
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAP1680

    Plasmid

    #137088

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFP

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    tagRFP
    

    Use

    Pcr template for donor dna

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH131

    Plasmid

    #48260

    Purpose

    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    GAL10/GAL1 bidirectional promoter
    

    Use

    Routine cloning vector

    Tags

    Expression

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    GAL10/GAL1 bidirectional

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-EAMP

    Plasmid

    #138265

    Purpose

    mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    iCas9 target-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-HTLV

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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