We narrowed to 14,950 results for: eng
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Plasmid#106077Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-182)-2xFv-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianMutationamino acids 1-182PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
Plasmid#31480DepositorInsertTraffic Light Reporter 1.1 (ani target)
UseLentiviralMutationmCherry has M9S, M16L to reduce background mCherr…Available sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pv6_MCPH1-TandemBRCT
Plasmid#250354PurposeExpresses tandem BRCT domain from MCPH1 fused to eGFP. Can be used to detect yH2AX at DNA damage sites.DepositorInsertMCPH1 tandem BRCT
TagsNLS, eGFP and biotintag, TEV cleavageExpressionMammalianPromoterCAGGSAvailable sinceFeb. 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NmCas9-FLAG
Plasmid#47872PurposeFLAG tagged NmCas9 with no NLS.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNmCas9
UseCRISPRTagsFLAGExpressionMammalianAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p426 25Q GAL
Plasmid#1187DepositorAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA
Plasmid#158972PurposeVector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neuronsDepositorInsertdSaCas9-VP64
UseAAV and CRISPRExpressionMammalianPromoterpMecp2Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI H2B-GFP
Plasmid#92399PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIRES H2B EGFP
ExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7
Plasmid#175529PurposeExpression of HaloTag7 at the inner membrane of mitochondria in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCox8a-HaloTag7
ExpressionMammalianAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1pr_v1
Plasmid#97081PurposeEncodes an sgRNA that creates a DSB at the promoter of human NEAT1 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA NEAT1pr_v1
UseCRISPRPromoterU6Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
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lentiCRISPR v2-sgSLC7A11/xCT-2
Plasmid#161819Purposeknock out SLC7A11/xCT in mammalian cellsDepositorInsertsolute carrier family 7 member 11 (SLC7A11 Human)
UseCRISPR and LentiviralAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY003 (pFnCpf1_delta Cas)
Plasmid#69974PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes
UseCRISPRExpressionBacterialMutationCas genes are deleted from locusAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330A-1x5
Plasmid#58769PurposeExpresses Cas9 nuclease and gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x7
Plasmid#58771PurposeExpresses Cas9 nuclease and gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x4
Plasmid#58774PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET FLII12Pglu-600u
Plasmid#13563DepositorInsertFLIPglu F16A Y12S-ECFP-D13R
TagsCitrineExpressionBacterialMutationF16A ECFP insertion in binding proteinAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSB-TRE-CRISPRoff-EF1A-TetOn
Plasmid#203355PurposeDrug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPRoff-TetOn
Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by