We narrowed to 6,990 results for: Cad

1. pMax_mOrange_A215C

   Plasmid

   #177828

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_A215C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A215C

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFast-Flag-hRad9

   Plasmid

   #222468

   Purpose

   Expresses hRad9 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad9 (RAD9A Human)
   

   Use

   Tags

   Flag epitope

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-EGFP-C1-HsRCK

   Plasmid

   #25033

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   Rck (DDX6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDFDuet-AtUBC8-S-AtUBA1-S

   Plasmid

   #222714

   Purpose

   Expression AtUBA1-S and AtUBC8-S in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Inserts

   AtUBC8 (UBC8 Mustard Weed)
   ATUBA1 (UBA1 Mustard Weed)
   

   Use

   Tags

   S-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFast-hRad1

   Plasmid

   #222471

   Purpose

   Expresses hRad1 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad1 (RAD1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV14_NOP58

   Plasmid

   #73063

   Purpose

   expression clone for human NOP58 with C-terminal 3xFLAG-tag

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   NOP58 ribonucleoprotein (NOP58 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-hOCT4 (Plath)

   Plasmid

   #17964

   Depositor

   Kathrin Plath

   Article

   Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

   Insert

   POU domain, class 5, transcription factor 1 (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

10. CMV10-3xFlag Skp2 WT

    Plasmid

    #81115

    Purpose

    mammalian expression of Skp2 WT

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (SKP2 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUF1-dCas9-GCN5

    Plasmid

    #122509

    Purpose

    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

    Depositor

    Lubin Jiang

    Article

    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

    Insert

    dCas9-GCN5
    

    Use

    CRISPR

    Tags

    3*FLAG

    Expression

    Mutation

    dCas9(D10A,H840A)

    Promoter

    PfHsp86

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV3Tag8li-hHP1BP3-HA

    Plasmid

    #165468

    Purpose

    Human HP1BP3 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSP64-hERG1a

    Plasmid

    #53051

    Purpose

    Expresses alpha subunit of hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-6P-1-hs-ALPS 1-ALPS2-EGFP

    Plasmid

    #187113

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSG00000101199 (ARFGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Stat1 beta Flag pRc/CMV

    Plasmid

    #8704

    Depositor

    Jim Darnell

    Article

    Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

    Insert

    STAT1 beta (STAT1 Human)
    

    Use

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pDACR3883 [rab-3p::HYlight-RA::let-858utr]

    Plasmid

    #207667

    Purpose

    Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

    Depositor

    Daniel Colón-Ramos

    Article

    Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    HYlight-RA [T152E]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-TRAIL-R2

    Plasmid

    #61383

    Purpose

    Expresses TRAIL receptor 2

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 2 (TNFRSF10B Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLIC-myc-GBAi W211A

    Plasmid

    #171754

    Purpose

    For the mammalian expression of myc-GBAi W211A

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101297

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-906 of alpha-Catenin

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNvT-MHC::mCh

    Plasmid

    #67943

    Purpose

    Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

    Depositor

    Ulrich Technau

    Article

    Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    mCherry
    

    Use

    Transgenic reporter in the sea anemone, nematoste…

    Tags

    Expression

    Mutation

    Promoter

    Myosin Heavy Chain1 (MyHC1)

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pFast-His6-hRad17

    Plasmid

    #222465

    Purpose

    Expresses hRad17 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hRad17 (RAD17 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid

    #49235

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-myc-LC3 (Atg8)

    Plasmid

    #24919

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    LC3 (MAP1LC3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA4-ATR-C

    Plasmid

    #53769

    Purpose

    mammalian expression of flag-tagged ATR C-terminal fragment

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal domain, aa 1398-2644*

    Promoter

    CMV

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. Arl6ip1-mEmerald

    Plasmid

    #186965

    Purpose

    Express Arl6ip1 with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    ARL6IP1 (ARL6IP1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4-ATR-N

    Plasmid

    #53767

    Purpose

    mammalian expression of flag-tagged ATR N-terminal fragment

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Proc Natl Acad Sci U S A. 2002 May 14;99

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal domain, aa 1-1409

    Promoter

    CMV

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-PuroDTK.Neo

    Plasmid

    #72307

    Purpose

    PiggyBac Cassette contains puromycin and TK genes

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

    Insert

    Puromycin-DTK-neo
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Feb. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMXs-hu-N-Myc

    Plasmid

    #50772

    Purpose

    retroviral expression of human N-Myc

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    N-myc (MYCN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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