164,852 results

1. pAAV-CMV-FLEX-SaCas9-U6-sgTh

   Plasmid

   #159901

   Purpose

   Mutagenesis of Th

   Depositor

   Larry Zweifel

   Insert

   Th (Th Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. punc503GFP

   Plasmid

   #64021

   Purpose

   503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expression

   Depositor

   Joachim Berger, Peter Currie

   Article

   Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

   Insert

   unc-45b: GFP (unc45b Zebrafish)
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   503-bp promoter of zebrafish unc-45b

   Available since

   May 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. g4s pD47

   Plasmid

   #117448

   Purpose

   Expression of Cyth1

   Depositor

   Richard Kahn

   Insert

   Cyth1 (CYTH1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MSCV-PIG-Cre

   Plasmid

   #50935

   Purpose

   retroviral expression of Cre recombinase

   Depositor

   Pier Pandolfi

   Article

   Chen et al Nature. 2005 Aug 4;436(7051):725-30.

   Insert

   Cre recombinase
   

   Use

   Cre/Lox and Retroviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-FLEX-ArchT-tdTomato

   Plasmid

   #28305

   Purpose

   Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV5

   Insert

   ArchT-tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

   Plasmid

   #89991

   Purpose

   Donor template for generation of SOX2-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

   Plasmid

   #107941

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R)
   

   Use

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R and K548R

   Promoter

   CAG

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMVR8.74

   Plasmid

   #22036

   Purpose

   2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

   Depositor

   Didier Trono

   Insert

   gag pol tat rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMVV102

   Plasmid

   #215308

   Purpose

   Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PTac

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMVV103

    Plasmid

    #215309

    Purpose

    Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-mCherry-FKBP-GEF(ARHGEF1)

    Plasmid

    #85152

    Purpose

    acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H6GB1tevP53SS(1-393)

    Plasmid

    #200311

    Purpose

    Bacterial expression of human superstable p53 as His6GB1tev fusion

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Flag-Smad4

    Plasmid

    #80888

    Purpose

    mammalian expression of Smad4

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SERPINE1-bio-His

    Plasmid

    #52077

    Purpose

    Expresses full-length Plasminogen activator inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SERPINE1 (SERPINE1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-28c(+)-YTHDF3

    Plasmid

    #102276

    Purpose

    For bacterial expression of His-tagged human YTHDF3

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. α‐eGFP

    Plasmid

    #160214

    Purpose

    Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

    Depositor

    Sandra Schmid

    Article

    Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

    Insert

    α‐eGFP (Ap2a2, Ap2a1 Mouse)
    

    Use

    Retroviral

    Tags

    eGFP at Hinge region

    Expression

    Mutation

    Brain-specific Insert

    Promoter

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Human, Synthetic)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-mNG-IRES-mScarlet-dMCP

    Plasmid

    #231089

    Purpose

    Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

    Depositor

    John Ngo

    Insert

    mNG-IRES-mScarlet-dMCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-HA-Ubiquitin-K6

    Plasmid

    #121151

    Purpose

    Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All Ks apart from K6 mutated to Rs

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT3-EF1aH-PIK3CA H1047R

    Plasmid

    #180031

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Promoter

    EF1a

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge