We narrowed to 59,421 results for: cloning

1. pBSR (mCherry-surrogateTarget-bsr)

   Plasmid

   #66950

   Purpose

   Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   blasticidin
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Expression

   Mammalian

   Promoter

   beta-actin

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMOD_C3013

   Plasmid

   #91100

   Purpose

   Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   GFP (+ BaeI site for GT donor cloning)
   

   Use

   Unspecified

   Promoter

   PvUbi2

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. STAGR_SAMScaffold_hH1

   Plasmid

   #102847

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert SAM_hH1
   

   Use

   Pcr template for stagr inserts

   Promoter

   U6

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. JN607: pMVP/SB-DEST

   Plasmid

   #121860

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-FRB-HA no-pA

   Plasmid

   #82594

   Purpose

   3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRB-HA (no pA)
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. KK402: pMVP/Zeo-DEST

   Plasmid

   #121859

   Purpose

   pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. IO201: pMVP (R4-R3) mCherry

   Plasmid

   #121732

   Purpose

   pMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   mCherry
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-SGTAP no-pA

   Plasmid

   #82599

   Purpose

   3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   SBP-TEV-2x protein G
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. BB2_EF

   Plasmid

   #98538

   Purpose

   empty BB2 for transcription unit cloning

   Depositor

   Diethard Mattanovich, Brigitte Gasser, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCVL SFFV Sbf-Sal linker

    Plasmid

    #50434

    Purpose

    Cloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).

    Depositor

    Andrew Scharenberg

    Article

    Boissel et al Nucleic Acids Res. 2013 Nov 26.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-Dam-myc no-pA

    Plasmid

    #82600

    Purpose

    3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamID

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    Dam-Myc (no pA)
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. IG401: pMVP (L1-R5) RIP

    Plasmid

    #121694

    Purpose

    pMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    RIP
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. STAGR_SAMScaffold_h7SK

    Plasmid

    #102846

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert SAM_h7SK
    

    Use

    Pcr template for stagr inserts

    Promoter

    H7SK

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. KJ801: pMVP/Hygro-DEST

    Plasmid

    #121858

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223 SCAF8_isoC_CRISPR resistant

    Plasmid

    #122466

    Purpose

    pDONR for Gateway cloning

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 isoform C CRISPR resistant (SCAF8 Human)
    

    Use

    Gateway cloning

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR221-WNT3

    Plasmid

    #79500

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WNT3 (WNT3 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-mCherry-FRB-HA no-pA

    Plasmid

    #82595

    Purpose

    3' entry vector containing HA epitope fused to C-terminus and mCherry fused to N-terminus of FRB without pA for rapamycin/rapalog induced dimerization

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    mCherry-FRB-HA (no pA)
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. JL302: pMVP/Puro-DEST

    Plasmid

    #121856

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMT-PT2A-Tdtomato-GFP-iRFP670

    Plasmid

    #111814

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Tdtomato
    GFP
    iRFP670
    

    Use

    Cloning intermediate

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-Ui4-eSIBR

    Plasmid

    #82586

    Purpose

    5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAi

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    human ubiquitin C promoter followed by intronically expressed eSIBR cassette
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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