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We narrowed to 63,044 results for: cloning

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Showing: 2901 - 2920 of 63044 results

  7. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  14. R777-E011 Hs.ARHGAP35

    Plasmid
    #70295
    Purpose
    Gateway ORF clone of human ARHGAP35 [NM_004491.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ARHGAP35 (ARHGAP35 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70295-plasmid-map-sequence-id-125744

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  19. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid
    #172506
    Purpose
    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Publication
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    DisCas7-11 bacterial expression vector with golden gate site for guides
    Expression
    Bacterial
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172506-plasmid-map-sequence-id-344928

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Showing: 2901 - 2920 of 63044 results