We narrowed to 51,448 results for: son

1. pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

   Plasmid

   #229743

   Purpose

   Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

   Depositor

   Shawn Ferguson

   Article

   Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   LIR2 mutant WTFI -> ATFA

   Promoter

   EF1A

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. NST3_pOpOn2.1

   Plasmid

   #228066

   Purpose

   Expresses NST3 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

   Depositor

   Charles Anderson

   Article

   Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

   Insert

   NAC SECONDARY WALL THICKENING PROMOTING FACTOR3, NST3 (NAC012 Mustard Weed)
   

   Use

   Dexamethasone inducible vector

   Expression

   Plant

   Promoter

   CaMV35S for LhGR and pOp6-minimal CaMV35S for NST3

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-LbCas12a-8xNLS

   Plasmid

   #209020

   Purpose

   Lentiviral vector expressing human codon-optimized LbCas12a with 8xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   LbCas12a-8xNLS-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJET-22-DExCon-mNeonGreen-R11a

   Plasmid

   #179897

   Purpose

   Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. px335 Slc1a3 nickase 1

   Plasmid

   #129410

   Purpose

   Cas9n (nickase) with sgRNA directed to the terminal exon of mouse Slc1a3 gene

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al Sci Rep. 2021 Mar 8;11(1):5412. doi: 10.

   Insert

   sgRNA directing Cas9n to the proximity of Slc1a3 STOP codon (Slc1a3 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

   Plasmid

   #70667

   Purpose

   Human mutant of E2F1 lacking amino acids 193-359

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 lacking residues 193 to 359 (E2F1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   lacks amino acids 193-359

   Promoter

   cmv

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_ZNF597_WT

   Plasmid

   #82894

   Purpose

   Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   ZNF597 (ZNF597 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. M3K5_HUMAN_D0

   Plasmid

   #79710

   Purpose

   This plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, Nicholas Levinson, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   M3K5 (MAP3K5 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. E2AK2_HUMAN_D0

   Plasmid

   #79712

   Purpose

   This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, Nicholas Levinson, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   E2AK2 (EIF2AK2 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. DMPK_HUMAN_D0

    Plasmid

    #79717

    Purpose

    This plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, Nicholas Levinson, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DMPK (DMPK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. KC1G1_HUMAN_D0

    Plasmid

    #79691

    Purpose

    This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, Nicholas Levinson, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G1 (CSNK1G1 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. CSK22_HUMAN_D0

    Plasmid

    #79704

    Purpose

    This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, Nicholas Levinson, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK22 (CSNK2A2 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

    Plasmid

    #70669

    Purpose

    Human mutant of E2F1 lacking amino acids 284 to 359

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 lacking residues 284 to 359 (E2F1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    lacks amin acids 284 to 359

    Promoter

    cmv

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pEMS1710

    Plasmid

    #29296

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple238 (TRH Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    June 10, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCMV-hERalpha

    Plasmid

    #101141

    Purpose

    Mammalian expression of full-length human estrogen receptor-alpha

    Depositor

    Elizabeth Wilson

    Article

    Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

    Insert

    estrogen receptor-alpha (ESR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-mDlx-GCaMP6f-Fishell-2

    Plasmid

    #83899

    Purpose

    GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-GFP-Gal8

    Plasmid

    #127191

    Purpose

    Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Insert

    GFP-Gal8 (LGALS8 Human)
    

    Tags

    Gal8 is fused to the c-terminus of GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-nSpCas9-TadA8e

    Plasmid

    #209044

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8e-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-nSpCas9-TadA8.20m

    Plasmid

    #209045

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8.20m-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-mDlx-GFP-Fishell-1

    Plasmid

    #83900

    Purpose

    GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    eGFP
    

    Use

    AAV

    Promoter

    mDlx

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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