We narrowed to 3,075 results for: eed

1. pGreenII 0229/ProRUBY:GOXL4-Citrine:NOSt

   Plasmid

   #175566

   Purpose

   pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-4

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

   Insert

   GOXL4
   

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   RUBY

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGreenII 0229/ProRUBY:GOXL3-Citrine:NOSt

   Plasmid

   #175565

   Purpose

   pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-3

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

   Insert

   GOXL3
   

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   RUBY

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGreenII 0229/ProRUBY:GOXL2-Citrine:NOSt

   Plasmid

   #175564

   Purpose

   pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-2

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

   Insert

   GOXL2
   

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   RUBY

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-gamma3/gamma2SIL

   Plasmid

   #121433

   Purpose

   GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG3/GABRG2 SIL (Gabrg2, Gabrg3 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Myc-gamma1/gamma2SIL

   Plasmid

   #121432

   Purpose

   GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIG1_UL136_33kda_Myc

   Plasmid

   #74957

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Myc tag (EQKLISEEDL)

   Expression

   Mammalian

   Mutation

   M63A M78A M80A M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mutpRL-TK 1x perfect to bulged siRNA

   Plasmid

   #40765

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to bulged target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCI-neo-RL-ZEB2 200bmut

   Plasmid

   #35538

   Depositor

   Greg Goodall

   Article

   Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

   Insert

   3'UTR of ZEB2 gene (ZEB2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   6 Mutations in the miR-200b seed regions of the Z…

   Promoter

   Available since

   June 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_Puro_Telo_v2

   Plasmid

   #195139

   Purpose

   ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   Telomere (RTEL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a_Puro_Telo_v1

    Plasmid

    #195138

    Purpose

    ~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    Telomere (RTEL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Use

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUT_mNF-Cas13d-Triplex

    Plasmid

    #227709

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d-Triplex
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pBactin-Nedd1-mNeonGreen

    Plasmid

    #196864

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mNeonGreen (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mitoTALECD for_atp1_1397CN

    Plasmid

    #186203

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG71 BDNF 3'UTR mut

    Plasmid

    #12054

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    BDNF 3'UTR mut (BDNF Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDR-Lenti-CBh.6-SOD1

    Plasmid

    #188460

    Purpose

    For knockdown-replacement of SOD1 gene (SOD1 KDR lentiviral transfer plasmid, Fig 4)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CBh

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG182 FGFR1 3'UTR mut

    Plasmid

    #12009

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N15 3'UTR mut (FGFR1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Promoter

    Available since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

19. proUBI10::VIH2-FL::FLAG-C696A

    Plasmid

    #238336

    Purpose

    proUBI10::VIH2-FL::FLAG-C696A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-C696A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. proUBI10::VIH2-FL::FLAG-K219A-D292A

    Plasmid

    #238331

    Purpose

    proUBI10::VIH2-FL::FLAG-K219A-D292A ubiquitous expression in Arabidopsis plants

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Mustard Weed)
    

    Use

    Tags

    3x Flag-tag

    Expression

    Plant

    Mutation

    AtVIH2-K219A-D292A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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