161,472 results

1. pDONR223-ABL1

   Plasmid

   #23939

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ABL1 (ABL1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS-memb-mCherry

   Plasmid

   #53750

   Purpose

   Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   2x membrane linker
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDule-tfmF A65V S158A

   Plasmid

   #85484

   Purpose

   Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32V F108W Q109M I159M with 65Val and 158Ala

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-HDAC6.ΔBuz-FLAG

   Plasmid

   #30484

   Depositor

   Tso-Pang Yao

   Article

   Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

   Insert

   HDAC6 (HDAC6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of the C-terminal Ubiquitin binding doma…

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL hygro H-Ras V12

   Plasmid

   #18749

   Depositor

   Scott Lowe

   Article

   Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

   Insert

   H-Ras V12 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V12

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. PLX_307 GFP

   Plasmid

   #184492

   Purpose

   Lentivirus expression for GFP

   Depositor

   Todd Golub, Peter Tsvetkov

   Article

   Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Fis1

   Plasmid

   #182580

   Purpose

   Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   mCherry-Fis1
   

   Use

   Tags

   Fis1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT-EF-Pdgfralpha-EGFPN

   Plasmid

   #66787

   Purpose

   Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Eps15-pmCherryN1

   Plasmid

   #27696

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Eps15 (EPS15 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-iSeroSnFR-Nlgn (AAV9)

    Viral Prep

    #128485-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-iSeroSnFR-Nlgn (#128485). In addition to the viral particles, you will also receive purified pAAV-CAG-iSeroSnFR-Nlgn plasmid DNA. CAG-driven expression of a fluorescent reporter for serotonin (localized to dendrites). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Promoter

    CAG

    Tags

    None

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

11. pMXs-ms-Klf2

    Plasmid

    #50786

    Purpose

    retroviral expression of mouse Klf2

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Klf2 (Klf2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. Orf3a-mCherry

    Plasmid

    #165138

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Orf3a (ORF3a codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-EF1a-NLuc-IRES-Puro

    Plasmid

    #130936

    Purpose

    PiggyBac vector for constitutive NanoLuc expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcs2-HRI-GFP-IRES-mCherry

    Plasmid

    #226099

    Purpose

    HRI stability reporter construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. IGF1R_pLX307

    Plasmid

    #98344

    Purpose

    Lentiviral expression of IGF1R

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    IGF1R (IGF1R Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CanlonicSF

    Plasmid

    #178342

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RUSH reporter-HMGB1-SBP-GFP

    Plasmid

    #172357

    Purpose

    Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

    Depositor

    Guido Kroemer

    Article

    Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

    Insert

    high mobility group box 1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    SBP-GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Loren Looger, Douglas Kim, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-Puro CAG PSMA nanobody T-CAR

    Plasmid

    #171962

    Purpose

    Donor plasmid for PSMA nanobody T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic anti-PSMA nanobody CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-LSSmOrange-CNA35

    Plasmid

    #61605

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with LSSmOrange

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    LSSmOrange and CNA35
    

    Use

    Tags

    His tag

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCM184-ParB2::eGFP

    Plasmid

    #87252

    Purpose

    Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB2
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. Flag-HA-USP33

    Plasmid

    #22601

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP33 (USP33 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTripZ RB1 WT-3XFLAG

    Plasmid

    #209070

    Purpose

    DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag

    Depositor

    Matthew Oser

    Article

    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

    Insert

    human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. piRFP-N3-Tyrosinase

    Plasmid

    #80152

    Purpose

    Melanosome marker

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    iRFP682

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. p5E-mfap4

    Plasmid

    #70052

    Purpose

    5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter
    

    Use

    Gateway 5' element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Pdest-EF1α-EGFP-32bp barcode polyA library

    Pooled Library

    #211610

    Purpose

    Barcoded retrovirus plasmid library for capturing the transcriptome and viral barcode sequences of individual cells to infer their identities and lineage information.

    Depositor

    Yuejun Chen

    Article

    You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

    Species

    Synthetic

    Use

    Retroviral

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA-HDAC6.DC-FLAG

    Plasmid

    #30483

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Catalytically inactive mutant (H216/611A)

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hSyn-somBiPOLES-mCerulean

    Plasmid

    #154945

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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