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Showing: 281 - 300 of 19160 results

  1. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  2. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  3. K406

    Plasmid
    #129764
    Purpose
    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 406
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129764-plasmid-map-sequence-id-263574

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  4. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  8. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid
    #196973
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    GFP and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196973-plasmid-map-sequence-id-388919

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  9. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

    Plasmid
    #196976
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST
    Depositor
    Lukas Kapitein
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    GST and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196976-plasmid-map-sequence-id-388922

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  16. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid
    #196972
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    6xHis and GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196972-plasmid-map-sequence-id-388918

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  19. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid
    #196974
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Article
    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    GST and mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196974-plasmid-map-sequence-id-388923

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  20. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid
    #196975
    Purpose
    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag
    Depositor
    Lukas Kapitein
    Article
    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    6xHis and SNAP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196975-plasmid-map-sequence-id-388920

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Showing: 281 - 300 of 19160 results