We narrowed to 5,724 results for: chia
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Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.shHmga2
Plasmid#32399DepositorInsertshRNA targetting Hmga2 (Hmga2 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR S72T variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB cdc42 QL
Plasmid#61636Purposemammalian expression of cdc42 constitutively active mutantDepositorInsertCdc42 (CDC42 Human)
UseTagsExpressionMammalianMutationQ61L, constitutively activePromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICH67131
Plasmid#153223PurposeLevel 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminatorDepositorInsertNos promoter, NPTII, Nos terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB rhoA QL
Plasmid#61632Purposemammalian expression of rhoA constitutively active mutantDepositorInsertrhoA (RHOA Human)
UseTagsExpressionMammalianMutationQ63L, constitutively activePromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDY118A
Plasmid#182958PurposepSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.DepositorInsertsCas9
Gam, Beta, Exo
I-scel
UseTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-E2-Crimson
Plasmid#117658PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-dCas13b-ADAR2DD
Plasmid#190913PurposeAddgene #103871 with 3x FLAG on N-terminal of dCas13DepositorInsert3X FLAG
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNAIIIB HA JNK
Plasmid#61630Purposemammalian expression of JNKDepositorInsertJNK1 beta1 isoform (MAPK8 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pAhpC-RBS- LacZ
Plasmid#190060PurposeLacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoterDepositorInsertlacZ
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC012 LwCas13a locus with nontargeting spacer
Plasmid#91904PurposeLwCas13a locus into pACYC184 with nontargeting spacerDepositorInsertLwCas13a with non-targeting spacer
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-FOXO3a TM delta CT
Plasmid#1797DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHAExpressionMammalianMutationT32A, S253A, S315A, delta CT (aa 468-673)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCellulose
Plasmid#36300DepositorInsertpCellulose
UseSynthetic BiologyTagsExpressionMutationPromoterTwo promoters from the E coli genome drive two di…Available sinceNov. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
TOPO tTR-KRAB-rtTA3-Luc
Plasmid#68449PurposeTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible geneDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseLuciferase; GmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB.FRB-3myc-KLC1a
Plasmid#134627PurposeMammalian expression of mouse KLC1aDepositorInsertKLC1a (Klc1 Mouse)
UseTagsFRB, 3mycExpressionMammalianMutationWTPromoterChicken Beta actinAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-AP1/beta1
Plasmid#218802PurposeMammalian expression o fhuman AP1 b1DepositorInsertAP1 b1 (AP1B1 Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a T32A DBM (H212R)
Plasmid#1793DepositorInsertFOXO3a (FOXO3 Human)
UseTagsHA (YDVPDYASL)ExpressionMammalianMutationT32A, S253A, S315A, H212RPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
UseTagsExpressionBacterialMutationPromoterlacUV5Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only