We narrowed to 87,731 results for: lias
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pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX
Plasmid#208694PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA2m
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorInsertDopamine receptor D1 (Drd1) (Drd1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR2 (CXCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
FH-Tet2-pEF
Plasmid#41710PurposeThis plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian systemDepositorInserttet methylcytosine dioxygenase 2 (Tet2 Mouse)
UseTagsFLAG and HAExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
GLP1R-DuET
Plasmid#213253PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGLP1R (GLP1R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR10-DuET
Plasmid#213197PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR10 (CCR10 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR6-DuET
Plasmid#213223PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR6 (CXCR6 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR1-DuET
Plasmid#213218PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR1 (CXCR1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCR3-DuET
Plasmid#213199PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCCR3 (CCR3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRD2-DuET
Plasmid#213228PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertDRD2 (DRD2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-T2A-GFP
Plasmid#193781PurposeTricistronic expression of dCas9-EGFP-BSDDepositorInsertdead Cas9
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterIn frame with existing Cas9 promoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
DRD1-DuET
Plasmid#213227PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertDRD1 (DRD1 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHRM2-DuET
Plasmid#213208PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCHRM2 (CHRM2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4.1-Myc-RNF8
Plasmid#221676PurposeMammalian expression of Myc-tagged human RNF8DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
GCGR-DuET
Plasmid#213250PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGCGR (GCGR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
GPR97-DuET
Plasmid#213294PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR97 (ADGRG3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEX-PK-hLC3
Plasmid#61458PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagyDepositorInsertPK-hLC3 (MAP1LC3B Human, Fission Yeast)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE_hygro_mRFP1_NRF2
Plasmid#136579Purposemammalian expression of mRFP1-tagged human NRF2DepositorInsertNRF2 (NFE2L2 Human)
UseRetroviralTagsN-mRFP1-FLAGExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-BFP
Plasmid#64323PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-EBFP2ExpressionMammalianMutationPromoterCBh and U6Available sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
GPR87-DuET
Plasmid#213293PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGPR87 (GPR87 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-SF2
Plasmid#99021PurposeMammalian expression of SF2DepositorInsertSF2 (SRSF1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMVp-dCas9-3xNLS-VP64 (Construct 1)
Plasmid#55195PurposeExpresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsVP64ExpressionMammalianMutationPromoterCMV/hUBCAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
ADRB3-DuET
Plasmid#213182PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADRB3 (ADRB3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-osTIR1
Plasmid#207840PurposeInducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsosTIR
mCherry
mAID_-VHH-GFP4
UseTags3X HA-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FOXM1b
Plasmid#68811PurposeDoxycycline inducible lentiviral vector for FOXM1b expression in mammalian cellsDepositorInsertFOXM1b (FOXM1 Human)
UseLentiviral; Doxycycline inducibleTagsHAExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX
Plasmid#208692PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_gDA3m
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab8a-CA (Q67L)
Plasmid#101050PurposeExpresses HA-tagged human Rab8-CA (Q67L) in mammalian cellsDepositorInsertRab8a (RAB8A Human)
UseTagsHAExpressionMammalianMutationQ67LPromoterCMVAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
APJ-DuET
Plasmid#213185PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertAPJ (APLNR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
