167,601 results
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CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6
Plasmid#190162PurposeExpresses 24 Broccoli-tagged beta-actin mRNA in mammalian cellsDepositorInsertmcherry_(3'UTR, No Actin)_24Broccoli
Tags24×BroccoliExpressionMammalianPromoterCMVAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-eFFly-mCherry
Plasmid#104833Purpose3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detectionDepositorInsertseFFly
mCherry
UseLentiviral and LuciferaseExpressionMammalianAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_IRES_tdTomato
Plasmid#184046PurposeExpresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequenceDepositorInsertsEnhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site
ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRISPaint Gene Tagging Kit
Plasmid Kit#1000000086PurposeModular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tagsDepositorAvailable SinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSFV3
Plasmid#92072PurposeEmpty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.DepositorTypeEmpty backboneUseEmpty expression plasmid, for run-off transcripti…Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Syn61Δ3(ev5) ΔrecA (ev1)
Bacterial Strain#189857PurposeLaboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.DepositorBacterial ResistanceStreptomycinSpeciesSyntheticAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
prhaBAD-pA-Hia5
Plasmid#222303PurposePlasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertpA-Hia5
Tags6His and protein AExpressionBacterialMutationcodon optimized for bacterial expressionPromoterrhaBADAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2-PITCh
Plasmid#63670PurposeExpresses Cas9 nuclease and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon Nega
Plasmid#163117PurposeRed fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cellsDepositorInsertRed Glifon Nega
ExpressionMammalianPromoterCMVAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1wt-C1
Plasmid#131818PurposeExpresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYH9-WT-V5
Plasmid#183512PurposeGateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.DepositorAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-SQSTM1-WT
Plasmid#194582PurposeMammalian Expression of mEGFP-SQSTM1 WT Fusion ProteinDepositorAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)
Plasmid#71485PurposeBase plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon armsDepositorTypeEmpty backbonePromoterU6Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLYS1-FLAG-MitoGFP-HA
Plasmid#50057PurposeExpression of GFP targeted to mitochondria with Flag and HA tagsDepositorInsertMito-GFP
UseLentiviralTagsFlag and HAExpressionMammalianAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
LPC-flag-REST-WT
Plasmid#41903DepositorInsertRE1-silencing transcription factor (REST Human)
UseRetroviralTagsFLAGExpressionMammalianAvailable SinceMay 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLdCN2
Plasmid#125186PurposeExpresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in LeishmaniaDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaAvailable SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-dCas9-ZIM3
Plasmid#240527PurposeExpresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3DepositorInsertMMLVgag-dCas9-ZIM3
TagsFLAG, HAExpressionMammalianPromoterCMVAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA39-Wasabi
Plasmid#86116PurposemWasabi reporter gene gated by TlpA39DepositorInsertsTlpA39
mWasabi
Tags6xHisExpressionBacterialMutationD341A, D135V, A217V, L236FPromoterpTlpA and pTlpA, LacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-tag
Plasmid#55180PurposeFLAG-tagDepositorInsertFLAG
UseSynthetic Biology; Bacillus biobrick boxAvailable SinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_EGFPligand
Plasmid#79129PurposeLentiviral vector for surface expressed EGFP (SFFV promoter)DepositorInsertEGFP surface ligand
UseLentiviralAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) (AAV PHP.eB)
Viral Prep#164450-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) (#164450). In addition to the viral particles, you will also receive purified AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) plasmid DNA. minBetaGlobin-driven expression of Cre in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterminBetaGlobinAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tag5Amyc-GSK3b CA
Plasmid#16261DepositorAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RLUC POLIRES FLUC
Plasmid#45642PurposeBicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.DepositorInsertRLUC - IRES - FLUC
UseLuciferaseExpressionMammalianAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROP-sgRNA-MS2
Plasmid#153457PurposeCROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffoldDepositorInsertsgRNA empty backbone
UseCRISPRExpressionMammalianAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Integrin-Beta1-N-18
Plasmid#55064PurposeLocalization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610DepositorAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145
Plasmid#111731PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ145 (polyQ repeat)PromoterCMV and P10Available SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Venus-Akaluc
Plasmid#209768PurposeVenus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).DepositorInsertVenus-Akaluc
UseLuciferase; Piggybac transposonExpressionMammalianPromoterCAGAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
sAJM.1504
Bacterial Strain#108251PurposeMarionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10BDepositorBacterial ResistanceChloramphenicolAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-mTSPAN4-mCherry
Plasmid#210330PurposeOverexpression of mTSPAN4DepositorAvailable SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only
