We narrowed to 13,452 results for: Mpl;

1. pD649-HAsp-LAIR2-COMP5AP-AviTag-9xHis

   Plasmid

   #157085

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   LAIR2 (LAIR2 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-sTagRFP-TUBA1B

   Plasmid

   #207769

   Purpose

   Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. shCTNNB1 # 1

   Plasmid

   #42543

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTY-EF1a-mKdm2b (del CxxC domain)-Flag

   Plasmid

   #61740

   Purpose

   Express CxxC domain deleted mouse histone demethylase Kdm2b

   Depositor

   Yi Zhang

   Article

   He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

   Insert

   Kdm2b (Kdm2b Mouse)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   delete aa 588-624

   Promoter

   EF1a

   Available Since

   Feb. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pD649-HAsp-ROBO4-COMP5AP-AviTag-9xHis

   Plasmid

   #157428

   Purpose

   Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   ROBO4 (ROBO4 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag Leucyl tRNA synthetase pRK5

   Plasmid

   #46341

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   leucyl tRNA synthetase (LARS1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pF144 pAcGFP1 SOD1G85R

   Plasmid

   #26405

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G85R

   Available Since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

8. MSCV-IRES-GFP ASXL1 (p.Y591X) 3x FLAG

   Plasmid

   #81022

   Purpose

   Cancer-associated ASXL1 truncation mutant in retroviral vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   p.Y591X truncation

   Promoter

   5’LTR

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

9. Tet-Inducible Human Caspase-9 Alpha

   Plasmid

   #194708

   Purpose

   Inducible Expression of Human Caspase-9 Alpha

   Depositor

   Hannah Rabinowich

   Article

   Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

   Insert

   Human Caspase-9 Alpha (CASP9 Human)
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Epac-S-H90

    Plasmid

    #170340

    Purpose

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Thomas Wassmer, Zita Balklava

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. hRAD18 dC2-EGFP

    Plasmid

    #68826

    Purpose

    Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

    Depositor

    Satoshi Tateishi

    Article

    Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

    Insert

    human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCAG-TWIN-STREP-FLAG-FLCN

    Plasmid

    #164981

    Purpose

    FLCN expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Tags

    2xSTREP FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pODN-H2BC11-mR2

    Plasmid

    #183866

    Purpose

    Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 homology arms with linker-mRuby2 (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mRuby2

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pODN-mR2-MYH10

    Plasmid

    #183869

    Purpose

    Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 homology arms with mRuby2-linker (MYH10 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-FUS_LC_12E

    Plasmid

    #98654

    Purpose

    Expresses MBP-tagged phosphomimetic FUS AA 1-163

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBTetBl-CLIP-Cep152

    Plasmid

    #136874

    Purpose

    Mammalian expression of the centrosomal protein Cep152 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep152 (CEP152 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. shBIRC5 # 2

    Plasmid

    #42554

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shBIRC5 (BIRC5 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid

    #84244

    Purpose

    Expresses GA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sa dCas9
    GID1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-EF1a-mTagRFP-T-CAAX

    Plasmid

    #167973

    Purpose

    AAVS1 targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFP-T-CAAX

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEBTet-SNAP-Cep97

    Plasmid

    #136800

    Purpose

    Mammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep97 (CEP97 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. shBIRC5 # 1

    Plasmid

    #42553

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shBIRC5 (BIRC5 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pGBT9-Hook3

    Plasmid

    #198525

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook3 (HOOK3 Human)
    

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid

    #89154

    Purpose

    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    

    Tags

    Intein and chitin binding domain

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Rab8-T22N(Flag) in pcDNA3.1neo

    Plasmid

    #46784

    Purpose

    expression of T22N Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A T22N (RAB8A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T22N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

26. APEX2-6xHp

    Plasmid

    #214411

    Purpose

    Lipid droplet localization of APEX2-6xHp for electron microscopy

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    6x Spastin aa 43-92 (APEX2 Human)
    

    Tags

    APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

    Plasmid

    #63139

    Purpose

    Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

    Depositor

    Titia Sixma

    Article

    Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

    Inserts

    Ring1b (Ring1 Mouse)
    Bmi1 (Bmi1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-109 and Residues 1-159

    Promoter

    tac

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

    Plasmid

    #115787

    Purpose

    Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-DIO-P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170328

    Purpose

    Double-floxed inverse Orientation V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. eGFP Depdc5 pLJM1

    Plasmid

    #46380

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Differs from Depdc5 isoform 1 by 10 amino acids …

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pFRT/FLAG/HA-DEST EIF2C1

    Plasmid

    #19887

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Eukaryotic translation initiation factor 2C, 1 (AGO1 Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #74261

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    CMV

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

33. myc-Atg14 (396-492)

    Plasmid

    #86755

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 396-492

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. myc-Atg14 (181-413)

    Plasmid

    #86754

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 181-413

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. myc-Atg14 (201-492)

    Plasmid

    #86751

    Purpose

    N-terminal myc-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    atg14 (ATG14 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    aa 201-492

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAd-Track HA PGC-1 alpha

    Plasmid

    #14427

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Last four amino acids of PGC-1a removed to make t…

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.1/NDUFV1/myc-His

    Plasmid

    #127490

    Purpose

    Expresses myc-tagged NDUFV1 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV1 (NDUFV1 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid

    #157715

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pEBTetBl-CLIP-Cep128

    Plasmid

    #136872

    Purpose

    Mammalian expression of the centrosomal protein Cep128 N-terminally fused to CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    CLIP-Cep128 (CEP128 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFL His-SDS22 / PP1gamma

    Plasmid

    #113511

    Purpose

    Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Inserts

    SDS22 (PPP1R7 Human)
    PP1gamma (PPP1CC Human)
    

    Tags

    6*His

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pcDNA3.3−3xFLAG−AGO2(S387A)

    Plasmid

    #220462

    Purpose

    Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Human AGO2 (AGO2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Serine 387 to alanine

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSICO-CPSF6-358-eGFP

    Plasmid

    #110693

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-eGFP (CPSF6 )
    

    Use

    Lentiviral

    Tags

    EGFP

    Mutation

    Truncation of CPSF6 isoform 2 at amino acid 358

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLJM1 FLCN (R164A) FLAG GFP

    Plasmid

    #164980

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Mutation

    R164A

    Promoter

    UBC

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pIRES:mTREK-1(G137I)

    Plasmid

    #133274

    Purpose

    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G137I

    Promoter

    CMVd2

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. FUW-TetO-Sox17

    Plasmid

    #128828

    Purpose

    dox-dependent expression of mouse Sox17

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Sox17 (Sox17 Mouse)
    

    Use

    Lentiviral

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pOPINO_H11

    Plasmid

    #184277

    Purpose

    Expresses nanobody H11 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11
    

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pEBTet-SNAP-Cep78

    Plasmid

    #136827

    Purpose

    Mammalian expression of the centrosomal protein Cep78 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Cep78 (CEP78 Human, Synthetic)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLenti-mitoX4-mCerulean

    Plasmid

    #185807

    Purpose

    Mitochondrial targetted (COX8) mCerulean

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    mito-mCerulean
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. Gal4-entry+NLS

    Plasmid

    #128013

    Purpose

    Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    Gal4 DBD-SV40NLS-3x-Flag
    

    Use

    Gal4 entry vector with nls to generate tethering …

    Tags

    Gal4-DBD-SV40NLS-3xFlag

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pmKO-imp α

    Plasmid

    #200082

    Purpose

    Expresses mKO-tagged human importinα in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

    Insert

    importin α (KPNA2 Human)
    

    Tags

    mKO

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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