We narrowed to 12,310 results for: LEA
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Plasmid#145755PurposeBacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-SUMO-AsCas12f-2NLS
Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
ExpressionBacterialAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CBS-7169
Plasmid#253633PurposeMpCas12a2 expressionDepositorInsertMpCas12a2 E. coli codon optimized C-terminal His tag
ExpressionBacterialMutationN/APromoterT7Available sinceMay 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-hDDI2_pET11a
Plasmid#169120Purposeexpression of proteinDepositorInserthDDI (DDI2 Human)
ExpressionBacterialAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT344_pattB-pCA-GFP-pA-FRT5
Plasmid#52550Purposefor Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing miceDepositorInsertsPhiC31o attB (full length)
GFP
ExpressionMammalianAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.28H.28TM.28.BB.3z
Plasmid#200680PurposeModular CD28hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
Plasmid#176534PurposeBacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrepDepositorInsertCRISPR-associated IscB (Delaware Bay metagenome)
Tags14xHis-MBP-bdSUMO and TwinStrepExpressionBacterialPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046814
Plasmid#145601PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTriEX-Antenna-GDI Rac1
Plasmid#83359PurposeExpression of Antenna-Rac1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRac1 (RAC1 Human)
TagsFRET Antenna (mCerulean-cpVenus) and HisExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSc1-DD
Plasmid#80439PurposeExpresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertssp Cas9 gRNA
eGFP
UseCRISPRTagsE. coli dihydrofolate reductaseExpressionMammalianPromoterCMV and U6Available sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
His-SUMO-Rad23A_pET11a
Plasmid#169124Purposeexpression of proteinDepositorAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pChimera
Plasmid#61476PurposeVector for conventional cloning that contains AtU6-26 promoter and sgRNA backboneDepositorInsertU6-26:sgRNA
UseCRISPRExpressionPlantPromoterU6-26Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
ExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Reb_221 aka PAS628
Plasmid#71233PurposeReb_1 with RebA(G90A)DepositorPublicationInsertReb locus
TagsnoneExpressionBacterialMutationRebA(G53A, G90A)Available sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
tetO-NR2F1
Plasmid#170698Purposedoxycycline-inducible overexpression of NR2F1DepositorInsertnuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
UseLentiviral; Doxycycline inducibleExpressionMammalianMutationdeletion of 36G- please see depositor commentsPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU422
Plasmid#178982Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)DepositorInsertsgRAMP
CRISPR array
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-2
Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EPB104
Plasmid#68369Purposecontains human U6 promoterDepositorAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_HA1m
Plasmid#190474PurposeExpress the medium-affinity histamine sensor GRAB_HA1m in neuronsDepositorInsertHistamine sensor GRAB_HA1m (medium affinity)
UseAAVPromoterhSynAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only