Addgene

We narrowed to 15,360 results for: LEA

Showing: 3161 - 3180 of 15360 results

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hrGFP-Keap1

Plasmid

#28025

Depositor

Qing Zhong

Article

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

Keap1 (KEAP1 Human)

Use

Tags

hrGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pFZ19

Plasmid

#36184

Depositor

Daniel Voytas

Article

Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

Type

Empty backbone

Use

Plant t-dna vector

Tags

Expression

Plant

Mutation

Promoter

XVE

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pclbw-mitoTagRFP

Plasmid

#58425

Purpose

Mammalian expression of tagRFP targeted to the mitochondrial matrix

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mito-tagRFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

cmv-bactin

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_K356D

Plasmid

#214312

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

K356D

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Reb_206 aka PAS629

Plasmid

#71234

Purpose

Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Use

Tags

mNeonGreen to second copy RebB, soluble mCherry a…

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE-600n

Plasmid

#13537

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Use

Tags

ECFP and Venus

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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TS module

Plasmid

#26021

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

Tension Sensor module

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-pgk-puro

Plasmid

#85747

Purpose

tetracyline-inducible lentiviral vector with no insert for use in mammalian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Type

Empty backbone

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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ZipGFP1-10_TEV

Plasmid

#81242

Purpose

Zipped GFP1-10 with TEV cleavage sequence T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

zipped GFP11 with TEV cleavage sequence T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP NFAT5 (no EGFP)

Plasmid

#13627

Depositor

Anjana Rao

Article

Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

Insert

NFAT5 (NFAT5 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pX330S-5

Plasmid

#58781

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pL0M-S-mTurquoise2-EC18152

Plasmid

#137074

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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pChimera

Plasmid

#61476

Purpose

Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

U6-26:sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6-26

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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p15a-AsCas12f-apmR

Plasmid

#171610

Purpose

AsCas12f1-based K. pneumoniae genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-300(HAT-)

Plasmid

#23254

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

H1415A E1423A Y1424A L1428S Y1430A H1434A

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

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pcNDA-NES-PhoCl2c-mCherry

Plasmid

#164037

Purpose

Optogenetic control of protein localization by PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NES-PhoCl2c-mCherry

Use

Tags

kozak sequence

Expression

Mammalian

Mutation

Promoter

CMV promotor

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-5-enzymatic-domain

Plasmid

#214306

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP5 (CASP5 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

aa133-434

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839)

Plasmid

#163509

Purpose

AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications.

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Has Service

AAV PHP.eB

Insert

H2B-SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_g5-HT2h

Plasmid

#208709

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cells

Depositor

Yulong Li

Article

Deng et al bioRxiv 2023.05.27.542566

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Use

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Promoter

Available since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

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