We narrowed to 23,077 results for: REP
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Plasmid#42251PurposeExpresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promotersDepositorInsertStreptococcus pyogenes Cas9
UseCRISPR; Zebrafish expressionPromoterCMVAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRL-SV40P
Plasmid#27163PurposeRenilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experimentsDepositorInsertSV40 promoter
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-LC3
Plasmid#21075PurposeMammalian expression of rat LC3 fused to mRFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
TagsmRFPExpressionMammalianMutationFirst ATG is not included.Available SinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (AAV5)
Viral Prep#111568-AAV5PurposeReady-to-use AAV5 particles produced from pZac2.1-GfaABC1D-mCherry-hPMCA2w/b (#111568). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-mCherry-hPMCA2w/b plasmid DNA. GfaABCD1-driven, expression of mCherry fused plasma membrane calcium pump hPMCA2w/b. These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherryAvailable SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1Available SinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_NE1m (AAV9)
Viral Prep#123308-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1m (#123308). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1m plasmid DNA. Synapsin-driven expression of GRAB-NE1m norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-Blast
Plasmid#83481PurposeLentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTADepositorInsertsSpCas9
Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
UseCRISPR and LentiviralExpressionMammalianMutationReplaced puromycin N-acetyltransferase on the ori…PromoterTight Tre and human phosphoglycerate kinase 1 pro…Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax
Plasmid#174820PurposeMammalian expression of SpCas9 PEmax prime editorDepositorInsertPEmax
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cmyc
Plasmid#16011Purposemammalian expression of human cmycDepositorInsertcmyc (MYC Human)
ExpressionMammalianAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 myc PGC-1 alpha
Plasmid#10974PurposeMammalian expression of PGC-1a with myc and his tagsDepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9
Plasmid#49330PurposeExpresses sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertsCas9
dU6-sgRNA
UseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
Plasmid#71237PurposeExpress sgRNA and dCas9-KRAB from 3rd generation lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A GFP
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable SinceJan. 2, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ai9
Plasmid#22799PurposeRosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.DepositorInsertCAG-Floxed tdTomato
UseMouse TargetingAvailable SinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMJ806
Plasmid#39312DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialPromoterT7Available SinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only