We narrowed to 7,591 results for: Lif;

1. pQE-80L 2xHis-LRRK2 Armadillo E240R

   Plasmid

   #207345

   Purpose

   Bacterial expression of LRRK2 Armadillo

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   LRRK2 Armadillo (LRRK2 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   E240R; Armadillo domain only (aa 1-552)

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE-80L 2xHis-LRRK2 Armadillo K439E

   Plasmid

   #207346

   Purpose

   Bacterial expression of LRRK2 Armadillo

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   LRRK2 Armadillo (LRRK2 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   K439E; Armadillo domain only (aa 1-552)

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE-80L 2xHis-LRRK2 Armadillo F283A

   Plasmid

   #207348

   Purpose

   Bacterial expression of LRRK2 Armadillo

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   LRRK2 Armadillo (LRRK2 Human)
   

   Use

   Tags

   2xHis

   Expression

   Bacterial

   Mutation

   F283A; Armadillo domain only (aa 1-552)

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anti-Uncx [N155/9R]

   Plasmid

   #206537

   Purpose

   Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Uncx (Mus musculus) recombinant Mouse monoclonal antibody (Uncx Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+FLAG-KrasnatQ61R

   Plasmid

   #206843

   Purpose

   To express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Glutamine 61 to Arginine

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV Flag-LRRK2 K17A/K18A/R1441G

   Plasmid

   #186013

   Purpose

   Flag-LRRK2 K17A/K18A/R1441G

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor's comments below.

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-O-FUW-PBX4-P2A-mCherry

   Plasmid

   #203864

   Purpose

   Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   PBX4 (PBX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-mCherry-KATNA1, V55D

   Plasmid

   #180651

   Purpose

   Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GGACAGCACUCCCUUGAAA

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA528 CHMP4C, residues 156-233, no Cys

   Plasmid

   #180642

   Purpose

   Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4C (CHMP4C Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   residues 316-366

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 IST1, residues 315-360, no Cys

    Plasmid

    #180643

    Purpose

    Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 315-360

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-THTAP NRBF2 MIT residues 1-111

    Plasmid

    #180621

    Purpose

    Bacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    NRBF2 (NRBF2 Human)
    

    Use

    Tags

    HIS-GST

    Expression

    Bacterial

    Mutation

    Residues 1-111

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-guide-puro mBltp1 (KIAA1109) - 1

    Plasmid

    #198489

    Purpose

    lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mBltp1 (4932438A13Rik Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-guide-puro mBltp1 (KIAA1109) - 2

    Plasmid

    #198490

    Purpose

    lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mBltp1 (4932438A13Rik Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-guide-puro mAtp6v1a-1

    Plasmid

    #198477

    Purpose

    lentiviral stable expression of mAtp6v1a gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mouse ATPv1a (Atp6v1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-guide-puro mAtp6v1a-2

    Plasmid

    #198478

    Purpose

    lentiviral stable expression of mAtp6v1a gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mouse ATPv1a (Atp6v1a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGBT-RP2.2

    Plasmid

    #189842

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBT-RP2.3

    Plasmid

    #189843

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBT-RP8.3

    Plasmid

    #189848

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Expression

    Mutation

    Promoter

    beta Actin

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. kanMX6-ins1

    Plasmid

    #195038

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-sgCtrl_EF1a-Puro-T2A-BFP

    Plasmid

    #195500

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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