We narrowed to 10,953 results for: AGA

1. Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168242

   Purpose

   "Rescue Rac expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-WT
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HH06-LP Clone 6 heavy chain

   Plasmid

   #192176

   Purpose

   Clone 6 heavy chain (HH06)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 6 heavy chain (HH06)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. myc-hRAD18 d6BD

   Plasmid

   #68832

   Purpose

   Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

   Depositor

   Satoshi Tateishi

   Article

   Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

   Insert

   c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCNL-C1_fibrillarin

   Plasmid

   #65709

   Purpose

   Expresses CNL-fibrillarin in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   fibrillarin (FBL Human)
   

   Use

   Luciferase

   Tags

   CNL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAAV asyn S87E

   Plasmid

   #36057

   Depositor

   Hilal Lashuel

   Article

   Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

   Insert

   alpha-synuclein S87E (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S87E

   Promoter

   CMV

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pLG1-puro-sgULK1-1

   Plasmid

   #109004

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ULK1-sgRNA #1 (ULK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

   Plasmid

   #108737

   Purpose

   Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Cre recombinase fragment N-terminus to aa270
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pClneoMyc MOB1 T74A

   Plasmid

   #47939

   Purpose

   expresses Myc tagged human MOB1 containing T74A mutation

   Depositor

   Yutaka Hata

   Article

   Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

   Insert

   MOB1A (MOB1A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T74A

   Promoter

   CMV

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

   Plasmid

   #163644

   Purpose

   Makes a lentivirus that expresses mNeonGreen and Cas9.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   mNeonGreen-P2A-Cas9
   

   Use

   Lentiviral

   Tags

   mNeonGreen-P2A

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-HA-FbxO10-pcDNA3.1-

    Plasmid

    #52508

    Purpose

    expresses human FbxO10 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO10 (FBXO10 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRG-2.1T-neo-sgTAF12(human)#4.4

    Plasmid

    #105590

    Purpose

    lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting human TAF12 HFD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCHKO_HPRT1_exon3_2_Lb

    Plasmid

    #155054

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

    Plasmid

    #108773

    Purpose

    Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa269
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

    Plasmid

    #108774

    Purpose

    Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa270 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pClneoMyc MOB1 T12A

    Plasmid

    #47936

    Purpose

    expresses Myc tagged human MOB1 containing T12A mutation

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T12A

    Promoter

    CMV

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pONL-C1_Rab11a

    Plasmid

    #65707

    Purpose

    Expresses ONL-Rab11a in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Rab11a (Rab11a Mouse)
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 HRPT2 136X

    Plasmid

    #11049

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 136X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pT2-7xTcf-NLS-ONL-CP

    Plasmid

    #65716

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TRIM27 gRNA (BRDN0001149307)

    Plasmid

    #77515

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM27

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM27 (TRIM27 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT2-7xTcf-NLS-YNL-CP

    Plasmid

    #65714

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmyc-GFP-TNRC6A-NLS/NES-mut

    Plasmid

    #42002

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A, F951A, F955A, I958A, …

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUAST-HA, Pbl-A

    Plasmid

    #69792

    Purpose

    Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    van Impel et al Development. 2009 Mar;136(5):813-22. doi

    Insert

    Pebble - Drosophila guanine nucleotide exchange factor
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Encodes Pbl (NP_729306.1) lacking last 9 amino ac…

    Promoter

    hsp70 promoter

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pAPM-D4 miR30-HDAC1 ts2

    Plasmid

    #115848

    Purpose

    HDAC1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HDAC1 shRNA (HDAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DF-UAS-spa-GCaMP6f

    Plasmid

    #67568

    Purpose

    Zebrafish expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    HIS-tag

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pT2-7xTcf-NLS-CNL-CP

    Plasmid

    #65715

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSC2

    Plasmid

    #91180

    Purpose

    T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting NCR genes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA

    Plasmid

    #108831

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO.1P MUTYH_1

    Plasmid

    #160785

    Purpose

    Suppress MUTYH

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shMUTYH_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 HRPT2 227X

    Plasmid

    #11050

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 227X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only