Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 45,354 results for: cha

Clear keywords
Showing: 6601 - 6640 of 45354 results

  1. pCMVtrunc sfCherry2ΔC4-Lifeact

    Plasmid
    #231557
    Purpose
    Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Tags
    sfCherry2
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231557-plasmid-map-sequence-id-459687

    Enlarge

  2. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid
    #225947
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S3E S17E S19E
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225947-plasmid-map-sequence-id-446849

    Enlarge

  3. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid
    #225948
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S3A S17A S19A
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225948-plasmid-map-sequence-id-446850

    Enlarge

  13. pFUSEss-CHIg-mG1_CH3-3

    Plasmid
    #105853
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH3 derived…
    Promoter
    hEF1-HTLV prom
    Available Since
    April 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    105853-plasmid-map-sequence-id-305783

    Enlarge

  16. pCEP4-myc-S (B.1.351 lineage / South African variant)

    Plasmid
    #169849
    Purpose
    Mammalian expression plasmid for S of SARS-CoV-2 (B.1.351 lineage / South African variant) with N-terminal c-myc tag
    Depositor
    Erik Procko
    Article
    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d
    Insert
    Protein S
    Tags
    HA leader and c-myc tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    169849-plasmid-map-sequence-id-329351

    Enlarge

  17. pFUSEss-CHIg-mG1_CH2-3

    Plasmid
    #105851
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH2.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH2 derived…
    Promoter
    hEF1-HTLV prom
    Available Since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    105851-plasmid-map-sequence-id-206013

    Enlarge

  18. pFUSEss-CHIg-mG1_CH1h-3

    Plasmid
    #105850
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH1 and hinge.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with CH1 and hin…
    Promoter
    hEF1-HTLV prom
    Available Since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    105850-plasmid-map-sequence-id-206012

    Enlarge

  19. pFUSEss-CHIg-mG1_h-3

    Plasmid
    #105854
    Purpose
    Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived hinge.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Front Immunol. 2018 May 23;9:1096. doi:
    Insert
    IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)
    Expression
    Mammalian
    Mutation
    hybrid heavy chain of mouse IgG1 with hinge deriv…
    Promoter
    hEF1-HTLV prom
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    105854-plasmid-map-sequence-id-206026

    Enlarge

  28. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

    Plasmid
    #225956
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Full-length CKAP4 with additional luminal domain …
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225956-plasmid-map-sequence-id-446863

    Enlarge

  29. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid
    #225939
    Purpose
    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    STIM1 (STIM1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225939-plasmid-map-sequence-id-446835

    Enlarge

  30. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid
    #225944
    Purpose
    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    ATL1 (ATL1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225944-plasmid-map-sequence-id-446838

    Enlarge

  36. An R57A, R59A CAAX pMT2

    Plasmid
    #206119
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206119-plasmid-map-sequence-id-415935

    Enlarge

  37. An R57A, R59A HA CAAX pMT2

    Plasmid
    #206126
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Oct. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    206126-plasmid-map-sequence-id-412911

    Enlarge

  38. pET-hMICU1EF

    Plasmid
    #199180
    Purpose
    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
    Depositor
    Ming-Feng Tsai
    Article
    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    MICU1 (MICU1 Human)
    Tags
    His6 and MBP, Thrombin site, TEV site
    Mutation
    D421A, E432K, F433W, F453W
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    199180-plasmid-map-sequence-id-403144

    Enlarge

  40. hTln1-R1R2-17b

    Plasmid
    #191427
    Purpose
    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria
    Depositor
    Ben Goult
    Article
    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901
    Insert
    Human Talin1 R1R2 domains (TLN1 Human)
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    contains 17AA insertion from inclusion of exon 17b
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    191427-plasmid-map-sequence-id-380511

    Enlarge

Showing: 6601 - 6640 of 45354 results