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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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167,605 results

Showing: 4981 - 5010 of 167605 results

  1. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  2. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

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  5. mTagGFP2-Rab5a-7

    Plasmid
    #56417
    Purpose
    Localization: Ras GTPase, Excitation: 483, Emission: 506
    Depositor
    Michael Davidson
    Insert
    Rab5a
    Tags
    mTagGFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56417-plasmid-map-sequence-id-484241

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  6. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid
    #111731
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q145 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    111731-plasmid-map-sequence-id-226895

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  9. sAJM.1504

    Bacterial Strain
    #108251
    Purpose
    Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B
    Depositor
    Christopher Voigt
    Article
    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038
    Bacterial Resistance
    Chloramphenicol
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. pWPXL

    Plasmid
    #12257
    Purpose
    2nd generation lentiviral transfer plasmid
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12257-plasmid-map-sequence-id-237089

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  13. ECFP-Nucleus-7

    Plasmid
    #55346
    Purpose
    Localization: SV40 NLS Triplet, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Tags
    ECFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55346-plasmid-map-sequence-id-182186

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  19. pPB-CAG-Venus-Akaluc

    Plasmid
    #209768
    Purpose
    Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    Venus-Akaluc
    Use
    Luciferase; Piggybac transposon
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209768-plasmid-map-sequence-id-417214

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  25. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    26S subgenomic promoter and IRES
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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  29. TFORF3472

    Plasmid
    #144948
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFATC3 (NFATC3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144948-plasmid-map-sequence-id-404528

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Showing: 4981 - 5010 of 167605 results