We narrowed to 12,110 results for: NSI
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Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
Tags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pT7-lox
Plasmid#190059PurposeExpress the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7DepositorInsertlox
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pT7-codA
Plasmid#190058PurposeExpress the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7DepositorInsertcodA
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pYjjZ-RBS- sfGFP
Plasmid#190054PurposeGFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoterDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pKatG-RBS- sfGFP
Plasmid#190053PurposeGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoterDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pOxyS-RBS- sfGFP
Plasmid#190052PurposeGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoterDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD410
Plasmid#178200PurposeExpression of sRok from the hns promoterDepositorInsertphns + srok
ExpressionBacterialPromoterhns promoterAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK10 SFFV-mCherry-SG(s70587-3xMS2)SG-EGFP (FLP-IN)
Plasmid#191165PurposeExpression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the coding sequence in mammalian cells. Similar to EK0438, but with MS2 sequences directly after the sensor sequence, rather than 3' UTRDepositorInsertmCherry:s70587-3xMS2:EGFP
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK2-mC-S
Plasmid#172428PurposeExpression of full-length, human GIRK4 with C-terminal mCherry StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1
Plasmid#184644PurposeRetroviral expression of BirA-mRap1DepositorInsertBirA-rap1
UseRetroviralTagsBirA FLAGExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14L
Plasmid#183063PurposePlasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locusDepositorInsertsmCherry
sfpHluorin
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastPromoterRET2, Synthetic promoter, and TDH3Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Flag-BirA-Linker-Myc-mRap1-I312R
Plasmid#184645PurposeRetroviral expression of BirA Rap1 I312RDepositorInsertBirA-rap1-I312R
UseRetroviralTagsBirA FLAGExpressionMammalianPromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_HbDHN2 (pBS0789)
Plasmid#185257PurposeFor the mammalian expression of the Para rubber tree protein DHN_HbDHN2. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDHN_HbDHN2
ExpressionMammalianAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DHN_HbDHN1 (pBS0788)
Plasmid#185256PurposeFor the mammalian expression of the Para rubber tree protein DHN_HbDHN1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDHN_HbDHN1
ExpressionMammalianAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Co-NF-kB
Plasmid#180197PurposeExpresses FLAG-tagged Capsaspora NF-kB protein in mammalian cellsDepositorInsertCo-NF-kB
TagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-1224PromoterCMVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0768
Plasmid#177058PurposeMoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0124
Plasmid#177056PurposeMoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0120
Plasmid#177052PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ash1-5p
Plasmid#169712PurposeBacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removedDepositorInsertAsh1 (ASH1 Budding Yeast)
Tags6xHisExpressionBacterialMutationTruncation including encoding amino acids 420-480…PromoterT7Available SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS-KRAB-dCas9
Plasmid#120356PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonExpressionMammalianMutationKlf4 [10-483]Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-KRAB-dCas9
Plasmid#120357PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonExpressionMammalianMutationKlf4 [1-483]Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 3'UTR KGA
Plasmid#110402PurposeLuminescence-based glutaminase KGA isoform 3'UTR-mediated RNA stability reporterDepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330.puro sgRNA KGA Stop1
Plasmid#110405PurposeCRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistanceDepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChromeT-GFP]
Plasmid#153539PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianPromoterSynAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfKitlga
Plasmid#140292PurposeExpress soluble His-tagged zebrafish Kitlga in insect cellsDepositorAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-MNX1wt-IDR
Plasmid#145248PurposeExpresses fusion of GAL4 DNA-binding domain and MNX1wt IDRDepositorInsertMNX1wt-IDR (MNX1 Human)
ExpressionMammalianAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
RA35
Plasmid#141126PurposeExpresses dimeric LacI repressor (LacI-11) under Rhl promoterDepositorInsertsTagsssrA degradation tagExpressionBacterialMutationtruncated/dimeric version of LacI (missing last 1…Promoterengineered rhl promoter (responds to C4-HSL) and …Available SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE Y165A
Plasmid#139428PurposeGST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged tyrosine 165 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE P199A
Plasmid#139427PurposeGST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged proline 199 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST AO7RE I137A
Plasmid#139319PurposeGST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteriaDepositorInsertAO7 aa 126-258 (RNF25 Human)
TagsGSTExpressionBacterialMutationChanged isoleucine 137 to an alaninePromoterT7 promoter-1Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJA105
Plasmid#128521PurposeExpresses amyloid human Lysozyme in Pichia pastorisDepositorAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-Ib
Plasmid#121896PurposePlasmid for making zebrafish apoA-Ib in situ probeDepositorInsertApoA1b (apoa1b Zebrafish)
Available SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoBb.2
Plasmid#121899PurposePlasmid for making zebrafish apoBb.2 in situ probeDepositorInsertApoBb.2 (apobb.2 Zebrafish)
UseUnspecifiedAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.3
Plasmid#121905PurposePlasmid for making zebrafish apoA-IVb.3 in situ probeDepositorInsertapoa4b.3 (apoa4b.3 Zebrafish)
UseUnspecifiedAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChromeT-GFP
Plasmid#123316PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterSynAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVa
Plasmid#121902PurposePlasmid for making zebrafish apoA-IVa in situ probeDepositorInsertApoA4a (apoa4a Zebrafish)
UseUnspecifiedAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-Ia
Plasmid#121895PurposePlasmid for making zebrafish apoA-Ia in situ probeDepositorInsertapoA-Ia (apoa1a Zebrafish)
Available SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
Tags6His-TEVExpressionBacterialPromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
Tags6His-TEVExpressionBacterialMutationP46APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only