We narrowed to 5,403 results for: puc

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pRE57I-GNAc

Plasmid

#170637

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, GNAc cassette, lacI

Expression

Bacterial

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pTn7xTS-mPlum

Plasmid

#117392

Purpose

Tn7 tagging vector pTn7xTS with mPlum expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGGF012

Plasmid

#48849

Purpose

Provides a sulfadiazine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::SulfadiazineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition sites in SulfR removed …

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-moxGFP

Plasmid

#207688

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pZH516

Plasmid

#102669

Purpose

Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Expression

Bacterial

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPR_full-site

Plasmid

#186277

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-3xHA-Zeo

Plasmid

#207740

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-3xHA

Plasmid

#207707

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199485

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al Nat Commun. 2024 Jun 12;15(1):5011. doi:

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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LI F-G Spec

Plasmid

#104521

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Spectinomycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTX1-RNA0

Plasmid

#126040

Purpose

In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

RNA0

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTW194b63

Plasmid

#136930

Purpose

Directed evolution of SpCas10

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

npuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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S5_EcoRV_Ef1a_AscI

Plasmid

#128469

Purpose

Ef1a promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Ef1a promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCas49

Plasmid

#82391

Purpose

rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

rbcL-GFP

Promoter

PccmK2

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTFAP2C-donor

Plasmid

#113824

Purpose

CRISPR donor plasmid to tag human transcription factor TFAP2C with GFP

Depositor

Kevin White

Insert

TFAP2C homology arms flanking EGFP-IRES-Neo cassette (TFAP2C Human)

Use

CRISPR

Mutation

rs6025024, hg38:20:56638660:TTT/-

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB8742

Plasmid

#126908

Purpose

Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

pGPD1 and pFBA1

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1075

Plasmid

#111093

Purpose

Expresses eGFP donor in pUC19

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

eGFP

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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AP582-2

Plasmid

#70049

Purpose

Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

mCherry::linker::H2B

Tags

Ollas and V5

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-C-miniTurbo-Zeo

Plasmid

#207736

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-miniTurbo

Plasmid

#207703

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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