We narrowed to 5,133 results for: puc

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pJK340

Plasmid

#71837

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59N) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to asparagine

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJK346

Plasmid

#71836

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRL439

Plasmid

#70258

Purpose

Source of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genes

Depositor

Peter Wolk

Article

Elhai et al Gene. 1988 Aug 15;68(1):119-38.

Insert

psbA promoter

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAD06 K. lactis telomerase RNA hairpin

Plasmid

#61895

Purpose

Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin (nt 229-273)

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
LI D-E 11GFP

Plasmid

#54430

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

FLAG tag + and short linker + superfolder GFP 11 domain

Use

Cloning vector

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D mOrange noATG

Plasmid

#54441

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mOrange without Start codon

Use

Cloning vector

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C N-NES KOZ a c1

Plasmid

#54444

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CPK17-N-terminal + NES for enhanced export

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D T-Saphire noATG

Plasmid

#54438

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

T-saphhire without Start codon

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D GFP noATG

Plasmid

#54436

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

sGFP without Start codon

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D Cerulean noATG

Plasmid

#54439

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP Cerulean without Start codon

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D mCherry noATG

Plasmid

#54440

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mCherry without Start codon

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D YFP noATG

Plasmid

#54437

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

YFP Venus without Start codon

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF004

Plasmid

#48845

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::D-AlanineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGA002

Plasmid

#48813

Purpose

Provides the AP3 promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

AP3 promoter

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by substit…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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CJ47 (U1 snRNA (U1;21d) 3G mutant)

Plasmid

#17776

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

U1 snRNA (U1;21d) (snRNA:U1:21D Fly)

Mutation

3G mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pILGFP4G

Plasmid

#104502

Purpose

Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

ScPGM2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4K

Plasmid

#104506

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpGAL1 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4O

Plasmid

#104510

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpdGAL2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4P

Plasmid

#104511

Purpose

Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SptGAL2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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