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We narrowed to 652 results for: weissman

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pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_2

Plasmid

#195133

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 2 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_1

Plasmid

#195132

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 1 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

Plasmid

#169191

Purpose

To express nsp10-nsp16 fusion protein in E. coli

Depositor

John Diffley

Article

Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

Insert

14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

Plasmid

#169177

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

Depositor

John Diffley

Article

Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

Insert

3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)

Use

Tags

3xFlag-His6 (nsp5-cleavable)

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

Plasmid

#169194

Purpose

Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells

Depositor

John Diffley

Article

Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

Insert

3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

3xFlag-His6

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBA407-Neo-COVGT5-mCherry

Plasmid

#201455

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT5_mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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DNA repair library with 1,573 sgRNAs

Pooled Library

#177663

Purpose

CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Species

Homo sapiens

Use

CRISPR

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only
DNA repair library with 336 sgRNAs

Pooled Library

#177664

Purpose

CRISPR-interference (CRISPRi) library of 366 sgRNAs targeting 118 genes encoding factors involved in DNA repair and associated processes

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Species

Homo sapiens

Use

CRISPR

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only
DNA repair library with 1,513 sgRNAs

Pooled Library

#177670

Purpose

CRISPR-interference (CRISPRi) library designed to target 476 genes involved in DNA repair and associated processes. It contains 1,513 sgRNAs and 60 non-targeting control sgRNAs.

Depositor

Brittany Adamson

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only
mTF-CRISPRai

Pooled Library

#122131

Purpose

Expresses gRNAs for mouse transcription factor-CRISPR activation

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only