We narrowed to 6,120 results for: nls
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Plasmid#222604PurposeExpression of MAC3-tagged GFP-NLS (C-terminal)DepositorInsertEGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 6, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-N-GFP-NLS
Plasmid#222607PurposeExpression of MAC3-tagged GFP-NLS (N-terminal)DepositorInsertEGFP and 3' nuclear localizaition sequence (NLS)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-mCh-Cry2WT_NLS
Plasmid#221925PurposeExpression of SV40 nuclear localization signal (NLS) fused to optogenetic protein mCh-Cry2WTDepositorInsertSV40 NLS
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of SV40 NLSPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LbCas12a-8xNLS
Plasmid#209020PurposeLentiviral vector expressing human codon-optimized LbCas12a with 8xNLSDepositorInsertLbCas12a-8xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-NLS-HA-TurboID
Plasmid#215073PurposeExpresses NLS-HA-TurboID in mammalian cells from a retroviral vector.DepositorInsertTurboID (birA )
UseRetroviralTagsNLS-HAExpressionMammalianMutationPromoterLTRAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-mAzGreen-Cyclin D1
Plasmid#215071PurposeExpresses NLS-mAzGreen-Cyclin D1 in mammalian cells from a retroviral vector.DepositorInsertCCND1 (CCND1 Human)
UseRetroviralTagsNLS-mAzGreenExpressionMammalianMutationPromoterLTRAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
NLS-mAzGreen-Cyclin D1 (HP)
Plasmid#215072PurposeExpresses NLS-mAzGreen-Cyclin D1 (HP) in mammalian cells from a retroviral vector.DepositorInsertCCND1 (CCND1 Human)
UseRetroviralTagsNLS-mAzGreenExpressionMammalianMutationCyclin D1(HP) denotes mutations M56A, V60A, and W…PromoterLTRAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 41kDa (BFP)
Plasmid#201392PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-BFP-2PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBP60g-NLS-3xmCitrine
Plasmid#192521PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertCALMODULIN BINDING PROTEIN 60g
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterCBP60gAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLipoP1-NLS-3xmCitrine
Plasmid#192522PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertPutative membrane lipoprotein (AT3G18250 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterLipoP1Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXPA10-NLS-3xmCitrine
Plasmid#192523PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertEXPANSIN A10 (EXPA10 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterEXPA10Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPIP1;4-NLS-3xmCitrine
Plasmid#192524PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertPLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterPIP1;4Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
LFNG:GFPnls-T2a-ER-Cre-ER
Plasmid#200107DepositorInsertLFNG:GFPnls-T2A-ER-Cre-ER
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWBhis03-Citrine-NLS
Plasmid#188558PurposeBinary vector for the expression of Citrine-NLS with MpIGPDm selective marker in Marchantia polymorpha (igpd mutants) .DepositorInsertCitrine-NLS and MpIGPDm
UseCRISPR; Entry vectorTagsExpressionMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-Halo-HSF2
Plasmid#200198PurposeExpression of full length HSF2 with SV40 NLSDepositorInsert3X SV40 NLS-Halo-HSF2 (Hsf2 Mouse)
UseTagsHaloExpressionMammalianMutationNonePromoterEF_1alphaAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.NWS_mCherry-NLS
Plasmid#178282PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.NWS and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCardinal-NLS
Plasmid#184450PurposeNucleus-targeted mCardinal expression in mammalian cellsDepositorInsertmCardinal-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-GAI
Plasmid#183405PurposeExpresses nuclear-localized LgBiT-GAIDepositorInsertLgBiT-GAI
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.S.V5_mCherry-NLS
Plasmid#178235PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.S.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.Ollas.V5_mCherry-NLS
Plasmid#178233PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.Ollas.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.Ollas.VSVg_mCherry-NLS
Plasmid#178232PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.Ollas.VSVg and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.Ollas.S_mCherry-NLS
Plasmid#178231PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.Ollas.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.NWS.V5_mCherry-NLS
Plasmid#178230PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.NWS.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.NWS.VSVg_mCherry-NLS
Plasmid#178229PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.NWS.VSVg and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.NWS.S_mCherry-NLS
Plasmid#178228PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.NWS.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.NWS.Ollas_mCherry-NLS
Plasmid#178227PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.NWS.Ollas and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HSV.V5_mCherry-NLS
Plasmid#178226PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HSV.V5 and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HSV.NWS_mCherry-NLS
Plasmid#178222PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HSV.NWS and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.HA.NWS_mCherry-NLS
Plasmid#178217PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.HA.NWS and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2MPEF-NLS-QUE37C
Plasmid#129326PurposeATP measurement, nucleus, Tol2 transposonDepositorInsertQUEEN-37C
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
YCe1903 HC_Kan_SV40-NLS_p6
Plasmid#100684Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertSV40-NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-wt-NLS
Plasmid#100788PurposeMammalian expression of Fluorescent CPI-17 nuclearDepositorInsertCPI-17-NLS (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-CPI-17-T38A-NLS
Plasmid#100780PurposeMammalian expression of CPI-17 neutral mutant nuclearDepositorInsertCPI-17-T38A-NLS (PPP1R14A Human)
UseTagsHAExpressionMammalianMutationT38APromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-CPI-17-T38E-NLS
Plasmid#100778PurposeMammalian expression of CPI-17 phospho-mimetic nuclearDepositorInsertCPI-17-T38E-NLS (PPP1R14A Human)
UseTagsHAExpressionMammalianMutationT38EPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
mKOet-PCNA-19-SV40NLS-4
Plasmid#57933PurposeLocalization: Nucleus, Excitation: 548, Emission: 561DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTagsmKOetExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hBEN-deltaNLS3
Plasmid#22161DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGST and HisExpressionMammalianMutationDeletion of amino acids 883-889PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51084-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51085-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV PHP.eB)
Viral Prep#192552-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomatoAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (AAV1)
Viral Prep#51082-AAV1PurposeReady-to-use AAV1 particles produced from AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato (#51082). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under the EF1a promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (AAV9)
Viral Prep#109048-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (#109048). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST plasmid DNA. Cre-dependent expression of the anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites, translated from an internal ribosomal entry site (IRES) following mRuby3 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby3Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51083-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato (#51083). In addition to the viral particles, you will also receive purified AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato plasmid DNA. Cre-dependent GCaMP6f calcium sensor and physically separate nuclear tdTomato, under control of the EF1a promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 NLS1 YFP
Plasmid#84912PurposeMammalian expresion of TDP-43 NLS1 YFPDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationK82A, R83A, K84APromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL103-SpCas9-FLAG-3XNLS
Plasmid#182032PurposeExpression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNPDepositorInsertStreptococcus pyogenes cas9 (cas9 Synthetic)
UseCRISPRTags12X His tag, 3XNLS, FLAG, and MBPExpressionBacterialMutationPromoterT7Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-GFP
Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas ServiceAAV PHP.eBInsertGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRE-mClover3-TDP-43ΔNLS
Plasmid#214917Purposeinducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear punctaDepositorInsertTDP-43ΔNLS (TARDBP Human)
UseLentiviralTagsmClover3ExpressionMutationmutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…PromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GW1-Peredox-mCherry-NLS
Plasmid#32381DepositorInsertPeredox-mCherry-NLS
UseTagsNuclear Localization SeqExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
NLS-QUE37C/pLBS CAG
Plasmid#129322PurposeATP measurement, nucleus, lentivirusDepositorInsertQUEEN-37C
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-EGFP
Plasmid#220594PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.DepositorInsertEGFP
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only