We narrowed to 8,279 results for: Dos

1. pGG438

   Plasmid

   #165486

   Purpose

   Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   D1135V, S1136R, D1332K, and R1333G mutations in S…

   Promoter

   CMV and T7

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-7Zf-wild

   Plasmid

   #23160

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Ribonuclease assay sub1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

3. AF0837

   Plasmid

   #19091

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Cell division protein pelota (DOM34 Archaeoglobus fulgidus)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pRB051_LEU2

   Plasmid

   #196993

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hPDGFRA-GFP

   Plasmid

   #22919

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest-Sumo

   Plasmid

   #106980

   Purpose

   LR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tag

   Depositor

   Han Choe

   Article

   Nguyen et al Sci Rep. 2017 Nov 23;7(1):16139. doi: 10

   Type

   Empty backbone

   Use

   Tags

   His8-Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIA10.7

   Plasmid

   #214451

   Purpose

   BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Inserts

   Tet-On 3G
   PuroR
   TRE3G CopGFP
   

   Use

   Bxbi donor

   Tags

   PuroR, T2A, and Tet-On 3G

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAM2_mCherry_GFP

   Plasmid

   #78172

   Purpose

   Doxycyline regulated expression of GFP in mammalian cells

   Depositor

   Timothy Kamp

   Article

   Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-Doxycycline

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAR4-CD4d3+4-bio

   Plasmid

   #73118

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   NAR4 (ART4 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Dom34(full length)

    Plasmid

    #19098

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-hGRM1-RFP

    Plasmid

    #22920

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-mA93-RFP

    Plasmid

    #22918

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    mouse riken gene A930038C07Rik promoter driving DsRedExpress (A930038C07Rik Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28b-Rv2416c_wild

    Plasmid

    #37649

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Rv2416c (eis Mycobacterium tuberculosis)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

14. Dom34+GST

    Plasmid

    #19099

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag, GST, and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

15. Ta1098

    Plasmid

    #19072

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. Dom34(1-138)

    Plasmid

    #19101

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

17. Dom34(265-386)

    Plasmid

    #19102

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. Dom34(265-377)

    Plasmid

    #19103

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. Tip alpha K102A/K104A (21-192)

    Plasmid

    #23159

    Depositor

    SeWon Suh

    Article

    Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

    Insert

    TNF-α-inducing protein
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed lysine 102 and lysine 104 to alanine, and…

    Promoter

    Available Since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only

20. Dom34 K82A

    Plasmid

    #19112

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    K82A

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only