We narrowed to 19,159 results for: met

1. pREF-GFP kan

   Plasmid

   #122061

   Purpose

   reporter plasmid for termination and terminator override to express GFP. Marked with URA3

   Depositor

   Daniel Reines

   Article

   Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

   Inserts

   intergenic terminator from yeast IMD2
   green fluorescent protein
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL1 comes in pYES2

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB2-TagRFP

   Plasmid

   #103997

   Purpose

   Plasmid encoding TagRFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   TagRFP
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (R32G, K42R, K67R, …

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmScarlet-H_peroxisome_C1

   Plasmid

   #85064

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Peroxisomal targeting sequence
   

   Use

   Tags

   mScarlet-H

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU1/Sm/3' Box_(GLuc)_TOP1

   Plasmid

   #68437

   Purpose

   Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP1 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U1

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mIFP24-C1

   Plasmid

   #54820

   Purpose

   Localization: C1 Cloning Vector, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson, Xiaokun Shu

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Type

   Empty backbone

   Use

   Tags

   mIFP24

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. Clover-Rab5a-7

   Plasmid

   #56530

   Purpose

   Localization: Ras GTPase, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Rab5a
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. mTagRFP-T-Vinculin-23

   Plasmid

   #58030

   Purpose

   Localization: Focal Adhesions, Excitation: 555, Emission: 584

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mTagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 4, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pET28a-ybbR-HIS-sfGFP-DocI

   Plasmid

   #58708

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   sfGFP-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE2-Hyg-omMx1

   Plasmid

   #52420

   Purpose

   Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

   Depositor

   Bertrand Collet

   Article

   Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

   Insert

   Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE2

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mRuby2-Cx43-7

    Plasmid

    #55894

    Purpose

    Localization: Gap Junctions, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Cx43
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pUAST cyto roGFP2-Orp1

    Plasmid

    #64996

    Purpose

    for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIntegrase_11

    Plasmid

    #60582

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. e-ciCas9_pcDNA5

    Plasmid

    #100553

    Purpose

    Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    e-ciCas9
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-CT3G-ERP2

    Plasmid

    #175497

    Purpose

    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKMV-VioC

    Plasmid

    #65349

    Purpose

    systhesis of vioC gene in the violacein pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    vioC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEB1-mCerulean ME

    Plasmid

    #103971

    Purpose

    Plasmid encoding mCerulean ME

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean ME
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Yellow Cameleon-Nano30/pcDNA3

    Plasmid

    #51963

    Purpose

    mammalian expression of Yellow Cameleon-Nano30, an ultrasensitive Ca2+ indicator with Kd = 30 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano30
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCMV/3' Box_(GLuc)_TOP2

    Plasmid

    #68435

    Purpose

    Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP2 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. gRNA-mCtnnb1

    Plasmid

    #84542

    Purpose

    mCtnnb1 gRNA expressing vector

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mCtnnb1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge