We narrowed to 18,153 results for: Met;

1. YEp181-CUP1-His-Ubi

   Plasmid

   #99539

   Purpose

   Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

   Depositor

   Helle Ulrich

   Article

   Davies et al Methods Mol Biol. 2012;920:543-67. doi:

   Insert

   UBI3(1-76) (RPL40A Budding Yeast)
   

   Use

   Tags

   7xHis

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB8644

   Plasmid

   #126919

   Purpose

   Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtI and HMG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tubulin-mhYFP

   Plasmid

   #186525

   Purpose

   Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Monomeric hyperfolder YFP
   

   Use

   Tags

   Tubulin

   Expression

   Mammalian

   Mutation

   hfYFP-S147P/L195M/V206K

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCBB-oriT

   Plasmid

   #32556

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   oriT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

   Plasmid

   #175536

   Purpose

   Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Beta4Gal-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-LSSmGFP

   Plasmid

   #186542

   Purpose

   LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   araB

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKIKOrbsARCm

   Plasmid

   #46765

   Purpose

   E.coli genomic integration rbsAR locus

   Depositor

   Claudia Vickers, Lars Nielsen

   Article

   Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

   Type

   Empty backbone

   Use

   E.coli genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV:eGFP-CaM-uTEV1Δ(220-242)

   Plasmid

   #135461

   Purpose

   Mammalian expression of uFLARE protease component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   eGFP-CaM-uTEV1Δ
   

   Use

   Tags

   eGFP, V5

   Expression

   Mammalian

   Mutation

   S219V mutation improves stability and S153N impro…

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss, Tadhg Begley

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

    Plasmid

    #175534

    Purpose

    Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    IgKchL_HA-HaloTag7-myc_PDGFRtmb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-miRFP720

    Plasmid

    #197213

    Purpose

    Expresses the protein of miRFP720 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP720
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNL(CMV)Fluc/CMV/WPREdU3

    Plasmid

    #136881

    Purpose

    Reporter vector expression firefly luciferase

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIS.MTRIA-MCH

    Plasmid

    #184606

    Purpose

    For expression of a fluorescent sensor for melanin-concentrating hormone in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-MCH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET51b(+)_ALFA-tag-SNAP

    Plasmid

    #136628

    Purpose

    Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    EGFP-NbALFA
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUREF-EX

    Plasmid

    #199331

    Purpose

    A pEF-BOS-EX-derived expression vector that contains an SV40 promoter-driven puromycin resistance gene

    Depositor

    Rikiro Fukunaga

    Article

    Fujii et al Genes Cells. 2023 Mar 2. doi: 10.1111/gt

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSF11-wmiRFP670-2-T2A-HO1

    Plasmid

    #197250

    Purpose

    Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP670-2-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCD/NL-BH∆1

    Plasmid

    #41791

    Purpose

    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    HIV-1 Gag/Pol and RRE sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-CitronRH

    Plasmid

    #158545

    Purpose

    Mammalian expression of nonbinding variant of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitronRH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO_b4g-HaloTag9

    Plasmid

    #175546

    Purpose

    Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET15bHis6Tnp

    Plasmid

    #79807

    Purpose

    Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

    Depositor

    Keji Zhao

    Article

    Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

    Insert

    His6Tnp
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV-OMM-jRCaMP1b

    Plasmid

    #127871

    Purpose

    Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCG-Hc∆18-AA-IL-13

    Plasmid

    #86171

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein displaying IL-13

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein fused to IL-13
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV.Syn.MTRIA-OT

    Plasmid

    #184594

    Purpose

    For production of AAV for a fluorescent sensor for oxytocin

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-OT
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

    Plasmid

    #55217

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRN12

    Plasmid

    #84456

    Purpose

    shuttle plasmid for sarA P1-mAmetrine expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mAmetrine
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CAG-E2-Crimson

    Plasmid

    #197196

    Purpose

    Expresses the protein of E2-Crimson in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    E2-Crimson
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJKR-H-cdaR

    Plasmid

    #62557

    Purpose

    Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Inserts

    beta lactamase
    sfGFP
    CdaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKIKOrbsARKm

    Plasmid

    #46768

    Purpose

    E.coli genomic integration rbsAR locus

    Depositor

    Claudia Vickers, Lars Nielsen

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCfB8787

    Plasmid

    #126921

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRVL-1

    Plasmid

    #104579

    Purpose

    Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Mouse CL kappa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG1244 p65-mCherry-FIREtag

    Plasmid

    #205175

    Purpose

    Expresses p65-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    p65-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSR prdm3 (PRDM16 shRNA)

    Plasmid

    #15505

    Depositor

    Bruce Spiegelman

    Article

    Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

    Insert

    PRDM16 shRNA (Prdm16 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pAB_PalkB_sfgfp_KanR

    Plasmid

    #166503

    Purpose

    Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PalkB

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCfB8748

    Plasmid

    #126918

    Purpose

    Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFlag-CMV2-Smyd2

    Plasmid

    #13521

    Depositor

    Shelley Berger

    Article

    Huang et al Nature. 2006 Nov 30. 444(7119):629-32.

    Insert

    Smyd2 (Smyd2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

37. pAG1238 PINK1-mCherry-FIREtag

    Plasmid

    #205174

    Purpose

    Expresses PINK1-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    PINK1-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ecAT3.10

    Plasmid

    #107215

    Purpose

    Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

    Depositor

    Mathew Tantama

    Article

    Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

    Insert

    ecAT3.10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJH136

    Plasmid

    #47554

    Purpose

    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

    Insert

    URA3
    

    Use

    Pcr template

    Tags

    Expression

    Mutation

    Promoter

    Native URA3 promoter, -243 to -1.

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge