Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

179,772 results

Showing: 3501 - 3520 of 179772 results

  6. pGL3 Basic Vector

    Plasmid
    #212936
    Purpose
    Firefly luciferase vector for investigating regions controlling transcription
    Depositor
    Debrya Groskreutz
    Type
    Empty backbone
    Use
    Luciferase
    Mutation
    WT
    Available since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    32 Articles
    212936-plasmid-map-sequence-id-419196

    Enlarge

  7. pCMV-GenEPi

    Plasmid
    #140236
    Purpose
    Mammalian expression of Piezo1-based fluorescent force sensor GenEPi
    Depositor
    Periklis Pantazis
    Publication
    Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
    Tags
    GCaMP 6s RS1 EF4
    Expression
    Mammalian
    Promoter
    simian CMV IE94
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    140236-plasmid-map-sequence-id-308737

    Enlarge

  10. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

    Plasmid
    #141379
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    13 Articles
    141379-plasmid-map-sequence-id-288091

    Enlarge

  11. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid
    #140002
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    140002-plasmid-map-sequence-id-275516

    Enlarge

  14. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    122650-plasmid-map-sequence-id-437776

    Enlarge

  15. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (AAV1)

    Viral prep
    #104493-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (#104493). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7b-WPRE plasmid DNA. Synapsin-driven, Cre-dependent jGCaMP7b expression. jGCaMP7b exhibits the brightest resting fluorescence and can be used for imaging of small neuronal processes (dendrites and axons). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV Retrograde)

    Viral prep
    #104492-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 3501 - 3520 of 179772 results