We narrowed to 2,757 results for: fas

1. pET-26b-NbVHH05-HA-His

   Plasmid

   #171567

   Purpose

   Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-HA-His (UBE2J1 Human)
   

   Use

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-GPHN-FingR-mGold2s-CCR5TC

   Plasmid

   #231785

   Purpose

   Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   GPHN-FingR-mGold2s-CCR5TC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Actin promoter with CMV enhancer

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMZ7

   Plasmid

   #223180

   Purpose

   pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

   Depositor

   Paul Blainey

   Article

   Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

   Type

   Empty backbone

   Use

   Chromosomal integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-ataxin-3

   Plasmid

   #89975

   Purpose

   Expresses GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mRFP-Ub

   Plasmid

   #11935

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin (RBX1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lenti SYN-FLAG-dCas9-VPR

   Plasmid

   #114196

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SYN

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-YR-ICUE-mGold2s

   Plasmid

   #231789

   Purpose

   Visualization of cAMP dynamics using YR-ICUE-mGold2s

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   YR-ICUE-mGold2s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPGK-T7/2-CD44v3 (fl)

   Plasmid

   #137814

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v3 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length and N280D in the V3 region

   Promoter

   PGK

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-YR-ICUE-mGold2t

   Plasmid

   #231790

   Purpose

   Visualization of cAMP dynamics using YR-ICUE-mGold2t

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   YR-ICUE-mGold2t
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-Ub KO

    Plasmid

    #11934

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin KO (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    All 7 lysines of ubiquitin mutated to arginines.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPGK-T7/2-CD44s (fl)

    Plasmid

    #137812

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length, I186T and I253V on CD44

    Promoter

    PGK

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pShuttle CMV RGECO-TnT

    Plasmid

    #124643

    Purpose

    Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

    Depositor

    Hugh Watkins, Matthew Daniels, Paul Robinson

    Article

    Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

    Insert

    RGECO-TnT (TNNT2 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAW-NbVHH05-mCherry

    Plasmid

    #171572

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-mCherry (UBE2J1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAW-Nb127D01-GFP

    Plasmid

    #171571

    Purpose

    Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-GFP (CXCR2 Human)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW-Nb127D01-mCherry

    Plasmid

    #171573

    Purpose

    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-mCherry (CXCR2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAW-CD8-Nb127D01-GFP

    Plasmid

    #171577

    Purpose

    Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    CD8-Nb127D01-GFP (CXCR2 Human)
    

    Use

    Tags

    CD8 and GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPGK-T7/2-CD44v6 (fl)

    Plasmid

    #137816

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R296K in the V6 region [Please see depositor comm…

    Promoter

    PGK

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPGK-T7/2-CD44v6-10 (fl)

    Plasmid

    #137820

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6-10 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length

    Promoter

    PGK

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPGK-T7/2-CD44v2-10 (fl)

    Plasmid

    #137824

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutations A282T, T483A, N535D, S5…

    Promoter

    PGK

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMS-dsRed2-WT LMNA-GFP minigene

    Plasmid

    #128230

    Purpose

    Lamin A splicing reporter

    Depositor

    Tom Misteli

    Article

    Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

    Insert

    lamin A (LMNA Human)
    

    Use

    Tags

    GFP and SV40-driven dsRed2 to identify transfecte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPGK-T7/2-CD44v8-10 (fl)

    Plasmid

    #137823

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v8-10 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length

    Promoter

    PGK

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pScarlessHD-C-3xVHH05-DsRed

    Plasmid

    #171580

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZac2.1 SV40-CMV-SaCas9-3xNLS

    Plasmid

    #78601

    Purpose

    AAV vector containing SaCas9

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    SV40-CMV-SaCas9-3xNLS
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40, CMV promoters

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

25. pScarlessHD-C-3x127D01-DsRed

    Plasmid

    #171578

    Purpose

    For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

    Plasmid

    #78603

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    U6-SaDMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

27. pScarlessHD-N-3xVHH05-DsRed

    Plasmid

    #171581

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAW-NbVHH05-GFP

    Plasmid

    #171570

    Purpose

    Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-GFP (UBE2J1 Human)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAW-CD8-NbVHH05-GFP

    Plasmid

    #171576

    Purpose

    Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    CD8-NbVHH05-GFP (UBE2J1 Human)
    

    Use

    Tags

    CD8 and GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pScarlessHD-N-3x127D01-DsRed

    Plasmid

    #171579

    Purpose

    For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3x127D01 (CXCR2 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPGK-T7/2-CD44v9 (fl)

    Plasmid

    #137818

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (fl) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length and mutation H48L

    Promoter

    PGK

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPGK-T7/2-CD44v6 (-cyt)

    Plasmid

    #137817

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region, R296K (please see dep…

    Promoter

    PGK

    Available since

    June 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCCLc-U6-shHMGA2.3-PGK-dTomato

    Plasmid

    #89606

    Purpose

    Silencing Hmga2 in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    shRNA targeting HMGA2 (HMGA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMS-dsRed2-C1824U LMNA-GFP minigene

    Plasmid

    #128231

    Purpose

    C1824U mutation Lamin A splicing reporter

    Depositor

    Tom Misteli

    Article

    Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

    Insert

    lamin A (LMNA Human)
    

    Use

    Tags

    GFP and SV40-driven dsRed2 to identify transfecte…

    Expression

    Mammalian

    Mutation

    C1824U

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

    Plasmid

    #89607

    Purpose

    Expression of LIN28A in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    LIN28A (LIN28A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MNDU3

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCCLc-U6-shHMGA2.2-PGK-dTomato

    Plasmid

    #89605

    Purpose

    Silencing Hmga2 in human cells

    Depositor

    Fernando Fierro

    Article

    Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

    Insert

    shRNA targeting HMGA2 (HMGA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pZac2.1 U6-SaAi9L-U6-SaAi9R

    Plasmid

    #78604

    Purpose

    AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    gRNAs for SaCas9 targeting Ai9 locus
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

38. pLEX307 GFP-Kif26b-C

    Plasmid

    #118483

    Purpose

    lentiviral expression of GFP-Kif26b-C in mammalian cells

    Depositor

    Henry Ho

    Article

    Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

    Insert

    mouse Kif26b-C (Kif26b Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Encodes GFP fused to amino acid 1735 to 2112 of m…

    Promoter

    EF1A

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPGK-T7/2-CD44s (-cyt)

    Plasmid

    #137815

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. UbG76V-eGFP-V5His_15aa

    Plasmid

    #23969

    Depositor

    Nico Dantuma

    Article

    Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

    Insert

    Ub-G76V-eGFP-V5HIS_15aa (UBB Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    G76V mutation in Ubiquitin. Ub fused to N-termin…

    Promoter

    Available since

    April 16, 2010

    Availability

    Academic Institutions and Nonprofits only