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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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ODD-Luciferase-pcDNA3

Plasmid

#18965

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

ODD-Firefly Luciferase (HIF1A Human)

Expression

Mammalian

Mutation

HIF1a ODD sequence fused in frame to luciferase O…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pCS2-MT GLI2 FL

Plasmid

#17648

Depositor

Erich Roessler

Article

Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

Insert

GLI2 (GLI2 Human)

Tags

6x Myc

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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Tag5Amyc-GSK3b WT

Plasmid

#16260

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

GSK3 beta (GSK3B Human)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 WT

Plasmid

#8974

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pAMPK alpha2 K45R

Plasmid

#15992

Depositor

Morris Birnbaum

Article

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Mutation

K45R

Promoter

CMV

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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Human V5-IRF3-pcDNA3

Plasmid

#32713

Depositor

Saumen Sarkar

Article

Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

Insert

IRF3 (IRF3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

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HER2 CA (V659E)

Plasmid

#16259

Depositor

Mien-Chie Hung

Article

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Expression

Mammalian

Mutation

Constitutively active: V659E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Beclin1

Plasmid

#21150

Depositor

Junying Yuan

Article

Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

Insert

PcDNA3-Beclin1 (BECN1 Human)

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV9)

Viral Prep

#83898-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Promoter

mDlx

Tags

mCherry

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
pFR_HCV_xb

Plasmid

#11510

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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mRFP-Clc

Plasmid

#14435

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Clathrin light chain (Clc Mouse)

Tags

mRFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pQCXI Puro DsRed-LC3-GFP

Plasmid

#31182

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV4-ApoE3

Plasmid

#87086

Purpose

Expresses ApoE3 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEGIP

Plasmid

#26777

Purpose

Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

EF1a-GFP-IRES-Puro (gfp )

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - HA-KLF4 FL

Plasmid

#34593

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSV Sport RXR alpha

Plasmid

#8882

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

RXR alpha (RXRA Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-flag S127A YAP

Plasmid

#27370

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Tags

flag

Expression

Mammalian

Mutation

S127A ( major phosphorylation site in YAP2)

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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FUCas9Cherry

Plasmid

#70182

Purpose

Expresses Cas9 with fluorescent Cherry reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

humanised S.pyogenes cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

hUbC

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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