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Showing: 7161 - 7200 of 7585 results

  1. pcDNA3.1 Flag YFP hNKCC1 A675C (NT441)

    Plasmid
    #50876
    Purpose
    Expresses human NKCC1 A675C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50876-plasmid-map-sequence-id-76349

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  2. pcDNA3.1 Flag YFP hNKCC1 I730C (NT504)

    Plasmid
    #50884
    Purpose
    Expresses human NKCC1 I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50884-plasmid-map-sequence-id-76357

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  3. pcDNA3.1 Flag YFP hNKCC1 A735C (NT509)

    Plasmid
    #50889
    Purpose
    Expresses human NKCC1 A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A735C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50889-plasmid-map-sequence-id-76362

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  4. pcDNA3.1 Flag YFP hNKCC1 S679C (NT447)

    Plasmid
    #50880
    Purpose
    Expresses human NKCC1 S679C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S679C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50880-plasmid-map-sequence-id-76353

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  5. pcDNA3.1 Flag YFP hNKCC1 N680C (NT468)

    Plasmid
    #50881
    Purpose
    Expresses human NKCC1 N680C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    N680C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50881-plasmid-map-sequence-id-76354

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  6. pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)

    Plasmid
    #50864
    Purpose
    Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A675C A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50864-plasmid-map-sequence-id-76337

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  7. pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)

    Plasmid
    #50869
    Purpose
    Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S679C I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50869-plasmid-map-sequence-id-76342

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  8. pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)

    Plasmid
    #50863
    Purpose
    Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50863-plasmid-map-sequence-id-76336

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  9. pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)

    Plasmid
    #50868
    Purpose
    Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I677C A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50868-plasmid-map-sequence-id-76341

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  13. pAAV-UBC-S1-GCaMP6f

    Plasmid
    #160722
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160722-plasmid-map-sequence-id-315521

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  14. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49077-plasmid-map-sequence-id-72205

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  21. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid
    #55613
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    Use
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    Silent mutations have been incorporated into the …
    Promoter
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55613-plasmid-map-sequence-id-86499

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  27. pcDNA3.1 Flag YFP hNKCC1 (NT17)

    Plasmid
    #49085
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Available since
    Nov. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    49085-plasmid-map-sequence-id-72211

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  28. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid
    #178057
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.
    Depositor
    Edward Boyden
    Article
    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    XRI-FLAG
    Use
    AAV
    Tags
    FLAG-MBP
    Expression
    Mammalian
    Mutation
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    178057-plasmid-map-sequence-id-349209

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  29. pAAV-UBC-S2-cAMPr

    Plasmid
    #160723
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter cAMPr (cAMP indicator), HA tag, and S2 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S2-cAMPr
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160723-plasmid-map-sequence-id-315524

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  36. pcDNA3.2-GJA1-20K-V5

    Plasmid
    #49859
    Purpose
    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    GJA1-20k (GJA1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    GJA1-20k fragment corresponds to residues 213-382…
    Promoter
    CMV
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-UBC-S3-ExRaiAKAR

    Plasmid
    #160724
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S3-ExRaiAKAR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160724-plasmid-map-sequence-id-315754

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  39. pAAV-UBC-S4-ExRaiCKAR

    Plasmid
    #160725
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-CKAR (PKC indicator), OLLAS tag, and S4 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S4-ExRaiCKAR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160725-plasmid-map-sequence-id-315528

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Showing: 7161 - 7200 of 7585 results