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Showing: 7281 - 7320 of 160936 results

  2. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  4. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  6. pLenti6.2-Nanoluc-ccdB

    Plasmid
    #87078
    Purpose
    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase
    Depositor
    Mikko Taipale
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Nanoluc luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87078-plasmid-map-sequence-id-173606

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  8. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV8)

    Viral Prep
    #105540-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    EGFP
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. AAV-hSYN-TdTomato

    Plasmid
    #193019
    Purpose
    AAV vector plasmid expressing TdTomato under the human synapsin (hSYN) promoter
    Depositor
    Bart Nieuwenhuis
    Insert
    TdTomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSYN
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    193019-plasmid-map-sequence-id-379935

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  17. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205247-plasmid-map-sequence-id-408440

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  20. pAAV-hSyn-EGFP (AAV1 trial size)

    Viral Prep
    #50465-AAV1.T
    Purpose
    Ready-to-use AAV1 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only

  24. ITGB1

    Plasmid
    #51920
    Purpose
    Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGB1 (ITGB1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 728, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51920-plasmid-map-sequence-id-79898

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  28. pCMV-CreEGFP

    Plasmid
    #165140
    Purpose
    Expression of Cre recombinase fused with EGFP in C-terminal
    Depositor
    Daekee Lee, David Threadgill
    Insert
    Cre recombinase
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    165140-plasmid-map-sequence-id-326583

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  30. AICSDP-7:SEC61B-mEGFP

    Plasmid
    #87426
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human SEC61B
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    SEC61B Homology Arms with mEGFP-linker (SEC61B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87426-plasmid-map-sequence-id-173967

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  34. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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  36. TFORF1334

    Plasmid
    #143791
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    POU3F3 (POU3F3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143791-plasmid-map-sequence-id-396244

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  40. pLVX-puro-cOVA

    Plasmid
    #135073
    Purpose
    Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance
    Depositor
    Maria Castro
    Insert
    cOVA (OVAL Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    135073-plasmid-map-sequence-id-402624

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Showing: 7281 - 7320 of 160936 results