We narrowed to 7,128 results for: SIM

Showing: 3641 - 3660 of 7128 results

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pAAV-DIO-dAPEX2

Plasmid

#117174

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHyg-yeGFP-His6

Plasmid

#212781

Purpose

C-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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AMPK-SPARK

Plasmid

#226255

Purpose

phases-of-separation based activity reporter of AMPK

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

AMPK-SPARK

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJAT17

Plasmid

#204298

Purpose

For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-EYFP

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCk2_spacer

Plasmid

#136072

Purpose

Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMA-SpCas9-g10

Plasmid

#80793

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCA17-CRISPR-Tag (11XTS1) for dSpCas9

Plasmid

#199448

Purpose

CRISPR-Tag sequence that can be efficiently bound by dSpCas9

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

CRISPR-Tag

Use

CRISPR

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP FAK

Plasmid

#50515

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Gu et al J Cell Biol. 1999 Jul 26;146(2):389-403.

Insert

protein tyrosine kinase

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAD-LyseR

Plasmid

#99244

Purpose

For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Expression

Bacterial

Promoter

beta-lactamase promoter

Available Since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available Since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE48

Plasmid

#90387

Purpose

IRF3/IRF7 - viral response gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF3/IRF7

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pQTEV-VPS28

Plasmid

#34803

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

vacuolar protein sorting 28 (yeast) (VPS28 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE2

Plasmid

#90336

Purpose

ATF6 - Unfolded protein response element (UPRE) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ATF6

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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p131CB10-tphKAB

Plasmid

#241911

Purpose

TPA catabolic operon under the control of a PCA inducible biosensor

Depositor

Neil Dixon

Article

Chacon et al Trends Biotechnol. 2025 Mar;43(3):647-67

Insert

tphK, tphA2, tphA3, tphB, tphA1

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMMP-LMP1-IRES-eGFP

Plasmid

#36955

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

LMP1 (LMP-1 )

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-GFP mAb

Plasmid

#214955

Purpose

Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-GFP mAb heavy chain
VNp-anti-GFP mAb light chain

Tags

VNp6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pJH001

Plasmid

#67641

Purpose

High copy Cas9 expression vector with LEU2 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
Empty

Use

CRISPR

Expression

Yeast

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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GCaMP-AMPK-SPARK

Plasmid

#226257

Purpose

Dual reporter of Ca2+ levels and AMPK activity

Depositor

Roland Malli

Article

Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

Insert

GCaMP-AMPK-SPARK

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pWZL-Blast-Flag-HA-hTERT

Plasmid

#22396

Depositor

William Hahn

Article

Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

Insert

TERT (TERT Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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