We narrowed to 9,800 results for: otos

1. pAAV-4mtD3cpv

   Plasmid

   #181874

   Purpose

   Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   4mtD3cpv
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   the 4mtD3cpv CDS differs from the recorded GeneBa…

   Promoter

   CaMKIIa

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOP22-5'UTR-BBa_B0034

   Plasmid

   #216626

   Purpose

   NanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTR

   Depositor

   Henrike Niederholtmeyer

   Article

   Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

   Insert

   T7p-5'B0034-NanoLuc-3'TMV
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOP01-UTC-7.0

   Plasmid

   #216625

   Purpose

   NanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test construct

   Depositor

   Henrike Niederholtmeyer

   Article

   Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

   Insert

   T7p-5'g10-NanoLuc-3'TMV
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiPGK Puro DEST JNKKTR AA Clover

   Plasmid

   #90238

   Purpose

   Lentiviral vector to express JNK KTR AA (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (AA mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBItetDT480GFP

   Plasmid

   #96905

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline responsive and bidirectional

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-ASAP2s

   Plasmid

   #101276

   Purpose

   Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn (human synapsin)

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. dmBACCS2NS-IRES-dOrai-IRES-GFP

   Plasmid

   #72895

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai-IRES-GFP
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVU-coHPDL WT

   Plasmid

   #174128

   Purpose

   Expresses wild type codon-optimized HPDL in mammalian cells

   Depositor

   Michael Pacold

   Article

   Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

   Insert

   4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Codon optimized

   Promoter

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

   Plasmid

   #107726

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK322 (TOP2A-mAC (Hygro))

    Plasmid

    #140653

    Purpose

    TOP2A tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECE-M2-PPP1R12C wt

    Plasmid

    #31659

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Protein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pECE-M2-GPHN

    Plasmid

    #31665

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    Gephyrin (GPHN Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDicAID_nCas9-PmCDA-2A-NptII_ETR

    Plasmid

    #91695

    Purpose

    Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A for nickase Cas9

    Promoter

    PcUbi

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Myc-Rab35 WT

    Plasmid

    #47433

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 (RAB35 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-elavl3-Voltron

    Plasmid

    #119037

    Purpose

    Pan neuronal expression of Voltron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Insert

    Voltron
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBItetDMPKSGFP

    Plasmid

    #96903

    Purpose

    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 0 CTG repeats in exon 15 located

    Depositor

    Thomas Cooper

    Article

    Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    EGFP and human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Use

    Tetracycline responsive and bidirectional

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline responsive and bidirectional

    Available since

    Nov. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. aMHC-Puro Rex-Blast

    Plasmid

    #21231

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. dmBACCS2-IRES-dOrai-IRES-GFP

    Plasmid

    #72893

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEE065

    Plasmid

    #176822

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    704
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-human CSFI-R-L301S- Y969F-IRES-GFP

    Plasmid

    #133745

    Purpose

    retroviral expression of CSF1-R mutant

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF1-R (CSF1R Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    L301S- Y969F

    Promoter

    MSCV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. hGATA-1 K137R

    Plasmid

    #118353

    Purpose

    This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K137R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

    Plasmid

    #99229

    Purpose

    Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos2
    

    Use

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    A69T for primed conversion applications

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBabepuro3-p18Flag

    Plasmid

    #24933

    Depositor

    Lynda Chin

    Article

    Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

    Insert

    P18 (CDKN2C Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTriEx-mCherry-Zdk1-Rac1 Q61L

    Plasmid

    #81059

    Purpose

    mammalian expression of Zdk1 tagged Rac1 Q61L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    Rac1 Q61L (RHOA Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTriEx-mVenus-Zdk1-RhoA Q63L

    Plasmid

    #81046

    Purpose

    mammalian expression of Zdk1 tagged RhoA Q63L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    RhoA Q63L (RHOA Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

    Plasmid

    #105643

    Purpose

    Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

    Depositor

    Sandra Rieger

    Article

    Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

    Insert

    Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

29. pEE062

    Plasmid

    #176819

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    701
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCI-neo-myc-LC3-HA

    Plasmid

    #45244

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only