We narrowed to 5,844 results for: pCas

1. pZC3H10.1.0-gDNA

   Plasmid

   #176385

   Purpose

   Expresses spCas9 and gRNA sequence targeting ZC3H10

   Depositor

   Kevin White, Barbara Stranger

   Insert

   ZC3H10 gRNA
   

   Use

   CRISPR

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZIC3.1.0-gDNA

   Plasmid

   #176379

   Purpose

   Expresses spCas9 and gRNA sequence targeting ZIC3

   Depositor

   Kevin White, Barbara Stranger

   Insert

   ZIC3 gRNA
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTX2.1.0-gDNA

   Plasmid

   #176376

   Purpose

   Expresses spCas9 and gRNA sequence targeting OTX2

   Depositor

   Kevin White, Barbara Stranger

   Insert

   OTX2 gRNA
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNR2E3.1.0-gDNA

   Plasmid

   #176386

   Purpose

   Expresses spCas9 and gRNA sequence targeting NR2E3

   Depositor

   Kevin White, Barbara Stranger

   Insert

   NR2E3 gRNA
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRR1

   Plasmid

   #166096

   Purpose

   Gal inducible expression of Cas9 WT

   Depositor

   Rebecca Burgess

   Insert

   WT spCas9
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Gal-inducible

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)

   Plasmid

   #160634

   Purpose

   TU for wtGFP expression driven by 2x35s promoter.

   Depositor

   Diego Orzaez

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   2x35s

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pITI2

   Plasmid

   #166095

   Purpose

   Gal inducible expression of D10A-Cas9 nickase

   Depositor

   Rebecca Burgess

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9-D10A

   Promoter

   Gal-inducible

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPH3

   Plasmid

   #166098

   Purpose

   Gal inducible expression of D10A-Cas9 nickase

   Depositor

   Rebecca Burgess

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9-D10A

   Promoter

   Gal-inducible

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FtsZ-MEEVF

   Plasmid

   #74126

   Purpose

   Expresses FtsZ fused to the MEEVF peptide tag via a flexible linker

   Depositor

   Lynne Regan

   Insert

   FtsZ-MEEVF
   

   Tags

   C-terminal linker sequence with the MEEVF tag (se…

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pDGB3 alpha1 P35S:BFP:T35S (GB3416)

    Plasmid

    #160637

    Purpose

    TU for the constitutive expression of Blue Fluorescent Protein

    Depositor

    Diego Orzaez

    Insert

    BFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRR2

    Plasmid

    #166097

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pITI1

    Plasmid

    #166094

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGB3_alpha1 Pnos:P19:Tnos (GB2762)

    Plasmid

    #160632

    Purpose

    TU for expression of P19 driven by Nos promoter.

    Depositor

    Diego Orzaez

    Insert

    P19
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. hPLD3-sgRNA-Cas9_mcherry

    Plasmid

    #199342

    Purpose

    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-RFP

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. hPLD4-sgRNA-Cas9_mcherry

    Plasmid

    #199343

    Purpose

    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry

    Depositor

    David Nemazee

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    sgRNA: accagtagtatgaagccacg

    Promoter

    human U6

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-CAG-hCAS9-neo

    Plasmid

    #166026

    Purpose

    AAVS1 targeting vector carrying CAG-SpCas9-neo

    Depositor

    Kosuke Yusa

    Insert

    AAVS1-CAGCas9IRESneo
    

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. BII-gR-PnTW

    Plasmid

    #133356

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Tags

    HA-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CaSpeR dhd 3X FLAG

    Plasmid

    #113002

    Purpose

    P element vector for transposon insertion of Flag-tagged Drosophila dhd into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Insert

    dhd (dhd Fly)
    

    Use

    P insertion vector

    Tags

    FLAG

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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