We narrowed to 10,362 results for: Coli
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Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen1
Plasmid#78180PurposeExpresses rsGreen1 in E. coli strains.DepositorInsertrsGreen1
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEA02
Plasmid#172193PurposeSuicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattL
ExpressionBacterialAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SOMA
Plasmid#118935PurposeUsed to express the SOMA FRET sensor in E. coli.DepositorInsertSOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
ExpressionBacterialAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1
Plasmid#98150PurposeExpresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1
TagsHis6-TEVExpressionBacterialMutationWTPromoterT7Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
1BR9-mCherry
Plasmid#193864PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.DepositorInsertmCherry
Tags6xHis, GFP11, and R9ExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSPYngK-hp
Plasmid#48118PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertsignal peptide of YngK
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS
Plasmid#127365PurposeBinary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - CPX
Plasmid#89620Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertCytosolic Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A-R69A
Plasmid#127787PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32, Arg36 and Arg69 to AlaAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodB
Plasmid#89613PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase B
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T5-ASG_ClpXP
Plasmid#153299PurposeExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917DepositorInsertASG
UseSynthetic BiologyExpressionBacterialPromoterT5Available SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1-Pct5.1-crRNA(dadR)
Plasmid#191634PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)DepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-Gli1
Plasmid#87225PurposeExpress hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coliDepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTYF-1xGfaABC1D-tTA
Plasmid#19977DepositorInsertGfaABC1D-tTA (GFAP Human, E.coli)
ExpressionMammalianAvailable SinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET24a_UDP_Xase
Plasmid#73158PurposeExpresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669DepositorInsertpET24a
ExpressionBacterialAvailable SinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhB D101N
Plasmid#108695PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutationDepositorInsertrnhB
TagsGSTExpressionBacterialMutationD101N catalytic site mutationPromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreenF
Plasmid#78181PurposeExpresses rsGreenF in E. coli strains.DepositorInsertrsGreenF
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2_rpoA-U
Plasmid#190956PurposeTest the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
TagsHisExpressionBacterialPromoterT7Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-A66S_rpoA-U
Plasmid#190969PurposeTest the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3_rpoA-U
Plasmid#190960PurposeTest the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-E67A_rpoA-U
Plasmid#190973PurposeTest the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP
Plasmid#127363PurposeBinary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
TagsGS linker, NES, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-6xHis-ARL2
Plasmid#67474PurposeExpression of hArl2 with N-term His6 tag in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRExpressionBacterialPromoterBBa_J23119 and J3-BBa_J23117Available SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)
Plasmid#111255PurposeBroad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptideDepositorInsertTorT signal peptide
ExpressionBacterialPromoterPcAvailable SinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)
Plasmid#108195PurposeExpresses PdR-PCNA3(R112C/T180C) fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C) fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf-9E (XSB526)
Plasmid#78514PurposeConstruct for expressing His10 - SNAPf -9E in E. ColiDepositorInsertSNAPf
TagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9E added to the C-terminal of SNAPfAvailable SinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/S148A
Plasmid#110829PurposeBacterial expression of E.coli DHFR N23PP/S148A mutantDepositorInsertecDHFR
ExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSPNprM-hp
Plasmid#48120PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein NprM under control of the xylose inducible promoterDepositorInsertsignal peptide of NprM
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGS1011
Plasmid#63783PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection markerDepositorInsertStrep-tag II followed by TRP1 selection marker
UseE. coli cloning vectorTagsStrep-tag IIPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSPVpr-hp
Plasmid#48119PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein Vpr under control of the xylose inducible promoterDepositorInsertsignal peptide of Vpr
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pANY1
Plasmid#110949PurposeUniversal mini-vector for efficient cloning of DNA and expression of protein in E. coli.DepositorInsertccd B cassette
TagsHisExpressionBacterialPromoterT7Available SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSM15
Plasmid#101640PurposeEncodes the fluorescent protein EG-4 for constitutive expression in E. coli.DepositorInsertEG-4
Tags6XHisExpressionBacterialAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL3
Plasmid#67418PurposeExpression of hArl3 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.8
Plasmid#78177PurposeExpresses rsGreen0.8 in E. coli strains.DepositorInsertrsGreen0.8
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fta RNA polymerase
Plasmid#254269PurposeEncodes E. coli codon-optimized rpoA, rpoB, rpoC, and rpoZ from Flagellimonas taeanensis driven by an inducible T7 promoterDepositorInsertsrpoA
rpoB
rpoC
rpoZ
ExpressionBacterialPromoterT7Available SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pQE30::CLOMELEON:Strep
Plasmid#247989PurposeExpression of the indicator protein CLOMELEON in E. coli.DepositorInsertCLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
Tags6xHis and StrepExpressionBacterialMutationCFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T…PromoterT5-phage promoterAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only