We narrowed to 12,237 results for: LEA
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pRK1035
Plasmid#8832DepositorInsertTVMV protease
TagsHis and MBP (cleaved)ExpressionBacterialMutationsynthetic gene with codons optimized for E. coliAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDY0209 ACTB Nicking guide +48
Plasmid#179109PurposeACTB N-term Nicking guide 1 +48 guideDepositorInsertACTB N-term Nicking guide 1 +48 guide
UseCRISPRAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330S-3
Plasmid#58779PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SQT1659
Plasmid#78743PurposeHuman expression plasmid for AsCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCAGAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSP2443
Plasmid#72252PurposeHuman expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHSN6A01
Plasmid#50586PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nuclear cpGFP
Plasmid#215707PurposeExpresses cpGFP in the nucleusDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-NanoLuc
Plasmid#188247PurposeTemplate plasmid to transcribe mRNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseExpressionMammalianPromoterT7Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP
Plasmid#55645PurposeCre-on/Flp-on ChR2-EYFP under the Synapsin promoterDepositorHas serviceAAV5, AAV8, AAV9, and AAV RetrogradeInsertChR2(H134R)-EYFP
UseAAV; Cre on/flp on chr2-eyfpTagsEYFPExpressionMammalianPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn Con/Fon EYFP
Plasmid#55650PurposeCre-on/Flp-on EYFP under the Synapsin promoterDepositorHas serviceAAV8 and AAV RetrogradeInserteYFP with introns
UseAAV; Cre on/flp on eyfpExpressionMammalianPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mRNA-CleanCap-mNeonGreen
Plasmid#188248PurposeTemplate plasmid to transcribe mRNA encoding mNeonGreenDepositorInsertmNeonGreen
ExpressionMammalianPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-Rab7a-7
Plasmid#55389PurposeLocalization: Ras GTPase, Excitation: 433, Emission: 475DepositorAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-p38KTRmCerulean3
Plasmid#59149PurposeORF encoding p38 KTR mCerulean3 flanked by Gateway sequencesDepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
Available sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
nuclear cpVenus control
Plasmid#186790PurposeIdentical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligaseDepositorInsertnuclear cpVenus control
UseLentiviralExpressionMammalianAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLeAPS-GFP-blast
Plasmid#182230PurposeLentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgeneDepositorInsertGFP-P2A-Blasticidin
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS-membrane-ceruleanFP
Plasmid#53749PurposeContains membrane localized cerulean fluorescent protein in pCS expression vector.DepositorInsert2x membrane linker
TagsCeruleanExpressionMammalianPromotersimian CMV IE94Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mCerulean-PA-Rac1
Plasmid#22030PurposeExpression of photoactivatable Rac1DepositorPublicationInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X His and mCeruleanExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by
