We narrowed to 59,342 results for: cloning

1. pET15-MHL

   Plasmid

   #26092

   Purpose

   SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Sept. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. Syn-FLEX-MCS-WPRE-SV40 - GCH8

   Plasmid

   #230818

   Purpose

   AAV cloning vector for CRE-dependent expression under the Syn promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. mOrange in pBSDONR P4r-P2

   Plasmid

   #121529

   Purpose

   For making C-terminal mOrange fusions using multisite Gateway cloning

   Depositor

   Roger Innes

   Insert

   mOrange
   

   Expression

   Bacterial

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. sCFP in pBSDONR P4r-P2

   Plasmid

   #121530

   Purpose

   For making C-terminal sCFP fusions using multisite Gateway cloning

   Depositor

   Roger Innes

   Insert

   sCFP
   

   Expression

   Bacterial

   Available Since

   Aug. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-Flex-MCS-WPRE-SV40 - GCH26

   Plasmid

   #230827

   Purpose

   AAV cloning vector for CRE-dependent expression under the TRE promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CaMKII-Flex-MCS-WPRE-SV40 - GCH20

   Plasmid

   #230824

   Purpose

   AAV cloning vector for CRE-dependent expression under the CaMKII promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME Actin Vhh mCherry (JDW 1240)

   Plasmid

   #224499

   Purpose

   Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mCherry
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-DreERT2 (JDW 1344)

   Plasmid

   #224523

   Purpose

   A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DreERT2
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

   Plasmid

   #224536

   Purpose

   A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG- NLS-mScarlet-I3
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-3x-FLAG-Ubiquitin (JDW 1098)

    Plasmid

    #224513

    Purpose

    A Gateway compatible middle entry clone containing a 3x FLAG tagged human Ubiquitin for monitoring ubiquitylation status

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3x-FLAG-Ubiquitin
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME Actin-Vhh sfGFP (JDW 1215)

    Plasmid

    #224495

    Purpose

    Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-sfGFP
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME EFS-rtTA/rtTA3 (JDW 1230)

    Plasmid

    #224525

    Purpose

    A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EFS-rtTA
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME Actin Vhh Halo Tag (JDW 1223)

    Plasmid

    #224498

    Purpose

    Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-Halo-Tag
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-3xNLS-(G2SG2)2-Cre (JDW 1149)

    Plasmid

    #224514

    Purpose

    A Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to Cre

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    3xNLS-(G2SG2)2-Cre
    

    Use

    Cre/Lox

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-EGFP-miR-MCS (JDW 1231)

    Plasmid

    #242562

    Purpose

    Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EGFP with artifial intron and MCS
    

    Use

    Gateway subcloning

    Available Since

    Sept. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-MCS (JDW 455)

    Plasmid

    #229832

    Purpose

    A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    attL1/L2 flanked MCS
    

    Use

    Gateway entry clone

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME nls EGFP WPRE bGH pA (JDW 488)

    Plasmid

    #224540

    Purpose

    A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    nls-EGFP-WPRE-bGH-pA
    

    Use

    Gateway cloning

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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