We narrowed to 11,256 results for: NSI

1. pCAG-OSF-KATNA1 R14A

   Plasmid

   #160052

   Purpose

   Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Tags

   One-Strep-Flag (OSF)

   Expression

   Mammalian

   Mutation

   R14A mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-OSF-KATNA1 V55D

   Plasmid

   #160053

   Purpose

   Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Tags

   One-Strep-Flag (OSF)

   Expression

   Mammalian

   Mutation

   V55D mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-π-EB1_IRES-mCherry

   Plasmid

   #197427

   Purpose

   Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-π-EB1_EF1α-mCherry

   Plasmid

   #197429

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-π-EB1_EF1α-EGFP

   Plasmid

   #197430

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   EGFP, GCN4 leucine zipper, LOV2, and Zdk1

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hTln1-R1R2-17b

   Plasmid

   #191427

   Purpose

   Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria

   Depositor

   Ben Goult

   Article

   Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

   Insert

   Human Talin1 R1R2 domains (TLN1 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   contains 17AA insertion from inclusion of exon 17b

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRetroX-TRE3G_NT-GSDMD_I105N_NoCys

   Plasmid

   #201177

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   aa1-276 only, I105N, C39A, C57A, C77A, C122A, C2…

   Promoter

   TRE3G

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRetroX-TRE3G_NT-GSDMD_I105N_C192only

   Plasmid

   #201178

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   aa1-276 only, I105N, C39A, C57A, C77A, C122A, C2…

   Promoter

   TRE3G

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRetroX-TRE3G_NT-GSDMD_NoCys

   Plasmid

   #201173

   Purpose

   Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D N-terminal domain (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   aa1-276 only, C39A, C57A, C77A, C122A, C265A, C19…

   Promoter

   TRE3G

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. H6GB1tev-IntC-P53(304-393)

    Plasmid

    #200315

    Purpose

    bacterial expression of P53 TETCTD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (304-393) (TP53 Human)
    

    Use

    Tags

    His6GB1tev and designed intein C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pROS_phleo-Tps1/Gsy2

    Plasmid

    #196612

    Purpose

    Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

    Plasmid

    #195504

    Purpose

    Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Coexpressed VLP NES

    Plasmid

    #182433

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, GAL10, and GAL7

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Coexpressed VLP NES (M)

    Plasmid

    #182435

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, GAL10, and GAL7

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Coexpressed VLP NES (G)

    Plasmid

    #182477

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, GAL10, and GAL7

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

    Plasmid

    #190154

    Purpose

    Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp2 Rat)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only rat Vamp3 AA #1-81

    Promoter

    MBP

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Linked Free NES (AG4TGGA)2

    Plasmid

    #182488

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Linked Free (PT)4P

    Plasmid

    #182490

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX459-dTLN1 intron

    Plasmid

    #176224

    Purpose

    A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX459-dTLN1 exon

    Plasmid

    #176033

    Purpose

    A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCSmChnERT(#99)

    Plasmid

    #184063

    Purpose

    cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

    Insert

    mCherry-nls-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLEX307_PDHA2S291D

    Plasmid

    #115193

    Purpose

    Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S291D

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLEX307_PDHA2S293D

    Plasmid

    #115194

    Purpose

    Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S293D

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLEX307_PDHA2S291D/S293D

    Plasmid

    #115195

    Purpose

    Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S291D/S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S291D/S293D

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLPC-FLAG.Mm.EPC1.delta.EPcA

    Plasmid

    #184655

    Purpose

    Retroviral expression of mouse EPC1 delta EPcA

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    epc1 delta EPcA

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT22iU66CriT(#387)

    Plasmid

    #184061

    Purpose

    cyclofen-inducible CRE activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLPC-Flag.Mm.Epc1-EPcA

    Plasmid

    #184657

    Purpose

    Retroviral expression of mouse Epc1 EPcA

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Epc1-EPcA

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPC-MYC.Mm.DMAP1

    Plasmid

    #184651

    Purpose

    Retroviral expression of mouse Dmap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Dmap1 (Dmap1 Mouse)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLPC-FLAG.Mm.EPC1.delta.EPcC

    Plasmid

    #184656

    Purpose

    Retroviral expression of mouse EPC1 delta EPcC

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Epc1 delta EPCc.QT

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. eSpCas9(1.1)-ABL1-gRNA1-exon4

    Plasmid

    #163322

    Purpose

    eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 1_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

33. eSpCas9(1.1)-ABL1-gRNA2-exon4

    Plasmid

    #163323

    Purpose

    eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 2_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

34. pNOC_dCas9_sgRNA Td1

    Plasmid

    #176257

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNOC_hfnCas12a-Nlux_crRNA NR2

    Plasmid

    #176245

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNOC_hfnCas12a_crRNA NR2

    Plasmid

    #176248

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNOC_hfnCas12a_crRNA NR1

    Plasmid

    #176247

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNOC_hfnCas12a-Nlux_crRNA NR3

    Plasmid

    #176246

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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39. pNOC_hfnCas12a_crRNA NR3

    Plasmid

    #176249

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR and Synthetic Biology; Expression in micro…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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40. 3xEGFP-mMeikin(1-391, E156R, R159E) - hRad21(142-275) - mMeikin(387-434)

    Plasmid

    #174712

    Purpose

    Mammalian expression of mouse Meikin with wild-type Separase cleavage site eliminated and C-terminal Separase cleavage site inserted

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Use

    Tags

    3xEGFP

    Expression

    Mammalian

    Mutation

    mMeikin: 1-391, E156R, R159E; hRad21: 142-275; mM…

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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