We narrowed to 4,107 results for: lenti crispr plasmid

1. pSECB

   Plasmid

   #106305

   Purpose

   Express spCas9 with sgRNA, blast resistance

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-PGK- dCas9-SunTag-P2A-HygR

   Plasmid

   #193137

   Purpose

   Expresses dCas9 fused to the SunTag scaffold

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   dCas9-SunTag-T2A-HygR
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

   Plasmid

   #67991

   Purpose

   CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
   

   Use

   CRISPR and Lentiviral

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGH224_sgRNA_2xMS2_Puro

   Plasmid

   #85413

   Purpose

   hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LES2A-CRE

   Plasmid

   #167933

   Purpose

   Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   mScarlet-P2A-CRE
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPapi

   Plasmid

   #96921

   Purpose

   expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

   Depositor

   David Root, John Doench

   Article

   Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-puro-v2

   Plasmid

   #127458

   Purpose

   Entry plasmid for guide cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-Guide-Zeo

   Plasmid

   #127173

   Purpose

   Entry plasmid for guide cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

    Plasmid

    #155305

    Purpose

    Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
    

    Use

    Lentiviral

    Tags

    2xNLS-3xFLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

    Plasmid

    #155307

    Purpose

    Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

    Plasmid

    #163138

    Purpose

    Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    PLK1 (PLK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

    Plasmid

    #232183

    Purpose

    Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6, hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. CSII-U6-gRNA-CBh-3xFLAG-PA-dCas9-P2A-Puro

    Plasmid

    #83306

    Purpose

    Lentivirus vector to express guideRNA and dCas9 with puro resistant gene

    Depositor

    Tohru Kimura

    Article

    Murakami et al Nat Immunol. 2021 Mar;22(3):301-311. doi

    Insert

    sgRNA and dCas9 from pX330
    

    Use

    Lentiviral

    Tags

    FLAG and PA tags

    Expression

    Mammalian

    Promoter

    U6 for sgRNA and CBh for dCas9

    Available Since

    Dec. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUGW-GFP-2-cut reporter

    Plasmid

    #131669

    Purpose

    Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    GFP-2-cut reporter
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-PGK-ABI-dCas9-P2A-mcherry

    Plasmid

    #121513

    Purpose

    expression of AB1-fused-dcas9 plasmids

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    AB1 and P2A-mcherry

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212962

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_non-targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212963

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_GFP targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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