We narrowed to 48,845 results for: des.1

1. pN1-RnKIF5C(1-560)-Halo-Flag

   Plasmid

   #188071

   Purpose

   Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

   Depositor

   Kristen Verhey

   Article

   Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

   Insert

   rat KIF5C (Kif5c Rat)
   

   Use

   Tags

   Flag and Halo

   Expression

   Mammalian

   Mutation

   aa1-560

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV-mCherry-hCdt1(1-100)ΔCy

   Plasmid

   #193759

   Purpose

   Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   mCherry-hCdt1(1-100)ΔCy (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   EF1a

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDUET-1-alpha-synuclein-mCerulean3-His6

   Plasmid

   #110061

   Purpose

   For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Tags

   6 x His and mCerulean3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

   Plasmid

   #173887

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FH1-PNGT#1-mCherry_PGK-H2B-CFP

   Plasmid

   #164103

   Purpose

   Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   PNGT#1-mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FH1-MGT#1-mVenus_PGK-H2B-CFP

   Plasmid

   #164100

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   MGT#1-mVenus
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-4T-1 3xHA-MKK4-EE

   Plasmid

   #47577

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK4-EE (MAP2K4 Human)
   

   Use

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Lacks the first 36 residues of the RefSeq protein…

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. TET-pLKO.1 PURO shID2 #2

   Plasmid

   #83087

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human ID2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

9. S1 1029 CP ABEMax strategy 1

   Plasmid

   #135357

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-6P-1 AUP1 292-410

    Plasmid

    #185331

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid

    #75409

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [D1_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Rat Alpha2 delta-1 mutMIDAS pMT2

    Plasmid

    #58730

    Purpose

    expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

    Insert

    alpha2 delta-1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MIDAS site mutations (D259VSGS to AVAGA)

    Promoter

    SV40

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LSB-hsa-miR-19b-1-5p

    Plasmid

    #103325

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-19b-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-19b-1-5p target (MIR19B1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Hook3(1-522)-SNAPf-Psc-StrepII

    Plasmid

    #111860

    Purpose

    C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    Hook3(1-552)-SNAPf (HOOK3 Human)
    

    Use

    Tags

    Psc, SNAPf, and Strep-II

    Expression

    Insect

    Mutation

    Optimised for expression in Sf9 cells

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUFlip-floxed2A-KalTA4-; gcry1:BFP -1

    Plasmid

    #173890

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LSB-hsa-miR-26a-1-3p

    Plasmid

    #103377

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-26a-1-3p target (MIR26A1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LSB-hsa-let-7f-1-3p

    Plasmid

    #103154

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7f-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7f-1-3p target (MIRLET7F1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tRNA-gRNA position [2_n-1] (GB1206)

    Plasmid

    #75407

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [2_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSB-hsa-miR-24-1-5p

    Plasmid

    #103374

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-24-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-24-1-5p target (MIR24-1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LSB-hsa-miR-30c-1-3p

    Plasmid

    #103413

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-30c-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-30c-1-3p target (MIR30C1 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge