We narrowed to 66,514 results for: des.1

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pLV hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#106249

Purpose

Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB T2A PuroR

Use

CRISPR and Lentiviral

Tags

HA-NLS and NLS-KRAB

Expression

Mammalian

Mutation

D10A and N580A

Promoter

hUbC

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV Fcgamma RIIa IRES neo

Plasmid

#14948

Depositor

Axel Nohturfft

Article

Trivedi et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Fcgamma RIIa (FCGR2A Human)

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pWT PolI

Plasmid

#11721

Depositor

Lawrence Loeb

Article

Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

Insert

DNA polymerase I (polA E. coli)

Expression

Bacterial

Mutation

wild-type

Available Since

Aug. 8, 2006

Availability

Academic Institutions and Nonprofits only

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sFlt1-i13 V5

Plasmid

#86044

Purpose

human Soluble Flt1 isoform i13 (terminates in intron 13)

Depositor

Christie Thomas

Article

Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

Insert

human soluble Flt1 isoform i13 (FLT1 Human)

Tags

6xHis and V5

Expression

Mammalian

Mutation

isoform i13 (terminates in intron 13)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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Col1A-5'probe

Plasmid

#20732

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1A-5'probe (Col1a1 Mouse)

Expression

Bacterial

Available Since

April 24, 2009

Availability

Academic Institutions and Nonprofits only

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EF1a_RUNX1_P2A_Hygro_Barcode

Plasmid

#120475

Purpose

Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

Plasmid

#183776

Purpose

AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

Cre

Use

AAV

Mutation

NA

Promoter

CAG

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTS117 His14-Avi-45xGS-anti GFP nanobody

Plasmid

#199370

Purpose

Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

anti-GFP nanobody

Tags

14xHis-Avi

Expression

Bacterial

Mutation

WT

Promoter

T5-LacO

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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PresentER-SIINFEKL (mCherry)

Plasmid

#102945

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Synthetic, Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only
pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Article

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_mmIrf1

Plasmid

#160098

Purpose

Expresses murine Irf1 in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Irf1 (Irf1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-wt

Plasmid

#33030

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Tags

6xMyc

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mLef1 (CT#1141)

Plasmid

#14021

Depositor

Cliff Tabin

Insert

Lef1 (Lef1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCMX-PGC1b

Plasmid

#1032

Depositor

Bruce Spiegelman

Article

Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

Insert

PGC-1 beta (Ppargc1b Mouse)

Tags

Gal4

Expression

Mammalian

Available Since

Oct. 7, 2005

Availability

Academic Institutions and Nonprofits only
p3xFLAG SARS-CoV2 RdRp.co

Plasmid

#154008

Purpose

Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

Depositor

Patrick Moore

Article

Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

Insert

Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)

Tags

3xFLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LiPOP2

Plasmid

#168266

Purpose

A single-component optogenetic tool for photoswitchable pyroptosis

Depositor

Yubin Zhou

Article

He et al Adv Sci (Weinh). 2021 May 6;8(13):210042

Insert

mGSDMD(1-276) (Gsdmd Mouse)

Tags

cpLOV2, mCherry

Expression

Mammalian

Mutation

R138A, K146A, R152A, R154A

Promoter

CMV

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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