We narrowed to 24,746 results for: CHI
-
Plasmid#48972PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianPromoterLTRAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP-WPRE
Plasmid#44352DepositorInsertEF1a-GFP
UseLentiviralPromoterhuman elongation factor -1a (pos 2526-3969)Available SinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046933
Plasmid#145592PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCTCON2-GYB
Plasmid#160927PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)DepositorInsertsBFP
sfGFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE
Plasmid#85930PurposeSensor PlasmidDepositorAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-RmMYB108
Plasmid#173183PurposeExpresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.DepositorInsertRmMYB108
ExpressionYeastAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM263-EcPPXc
Plasmid#38328DepositorInsertE. coli polyphosphatase (ppx Escherichia coli)
Tags6xHIS, GST, and TEVExpressionBacterialMutationC-terminus of E. coli polyphosphatase (described …PromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136463
Plasmid#149527PurposeBacterial expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti_CMV:Flag-CeNLuc_SV40:PuroR
Plasmid#183036PurposeExpresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAL612
Plasmid#119860Purposeprecusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 CysteineDepositorInsertouter membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
TagsnoneExpressionBacterialMutationCysterine 290 to Serine and Glycine 244 to Cystei…PromoterlacAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046763
Plasmid#145606PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOVC1_EcSSPB-nano_X
Plasmid#131212PurposeExpresses EcSSPB-nano (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-nano
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6
Plasmid#158147PurposeYeast display reporter for non-display yeast strains (wild-type construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLOT-syb-sp-YFP1-10
Plasmid#73700PurposeNeuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spYFP1–10 chimera
UseLexaop reporter vectorExpressionInsectAvailable SinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB5191
Plasmid#126911PurposePlasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiaeDepositorInsertX-4 and XII-5 gRNA
UseCRISPRAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-evoAmax-BlastR
Plasmid#152996PurposeC-to-T base editorDepositorInsertevoA-nSpCas9-UGI-UGI
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAID-CMV linearizing in pX330
Plasmid#140609PurposeCrispr/Cas9 plasmid for linearization of pAID-CMVDepositorInsertsgRNA for pAID-EF1a plasmid digestion
UseCRISPRAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFlag DVL2 (E499-G)
Plasmid#24803DepositorAvailable SinceJune 22, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
3xFNLDD/pCMV-7.1
Plasmid#48874PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
Tags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianPromoterCMV promoterAvailable SinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046764
Plasmid#145673PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
ExpressionBacterialPromoterProD, nTETO, and pBAD /AraCAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85857PurposeSensor PlasmidDepositorAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAL725
Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
TagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
E17TAG GFP zeo resistance
Plasmid#69118PurposeFor E17TAG GFP reporter expression in non-zeocin resistant strainsDepositorInsertE17TAG GFP
TagsHisExpressionBacterialMutationE17 to TAGAvailable SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
L1-A426D
Plasmid#15485DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKH-MoHyPer
Plasmid#78267PurposeExpress HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.DepositorInsertMoHyper
UseExpress in magnaportheExpressionYeastPromoterRp27Available SinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.shNkx2-1
Plasmid#32400DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101107PurposeEncodes dCas9(C)-VP64 with SAM components fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZA31
Plasmid#158509PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
Tags4xMycExpressionPlantMutationL17EAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-tLuc
Plasmid#107552PurposetLuc in AAV backboneDepositorInsertLuciferase
UseAAVAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
fADL-1e-flag-linker-p3
Plasmid#139441PurposeFor cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.DepositorInsertlinker with pelB-leader, cloning site, flag-tag and p3
ExpressionBacterialAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-Vinculin-23
Plasmid#55453PurposeLocalization: Focal Adhesions, Excitation: 433, Emission: 475DepositorAvailable SinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_NFIL3
Plasmid#86287PurposeDonor vector for 3' FLAG tag of human NFIL3DepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIL3_2
Plasmid#86349PurposeEncodes gRNA for 3' target of human NFIL3DepositorInsertgRNA against NFIL3 (NFIL3 Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIL3_1
Plasmid#86348PurposeEncodes gRNA for 3' target of human NFIL3DepositorInsertgRNA against NFIL3 (NFIL3 Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcB-His-CD)
Plasmid#176194Purposeexpresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
TagsGfcB-6xHisExpressionBacterialPromoterPtacAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-6xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
TagspelC leader-10xHisExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330(puro) Rosa26-H3F3B
Plasmid#73131PurposeExpression in Mammalian Cells, Cas9 Nuclease to analize translocationsDepositorInsertspCas9
TagsFlagExpressionMammalianAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-LexAOx4-Pmin-Cre
Plasmid#171759PurposeLentiviral vector for inducible Cre expression with 4x LexA operator (LexAO)DepositorInsertCre recombinase
UseLentiviralExpressionMammalianPromoterminimal promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP7
Plasmid#71470PurposeEncodes human full length SENP7. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 7 (SENP7 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
p7128 pHAGE-P-CMVt-N-HA-GAW-HLA-A
Plasmid#100154PurposeExpresses HLA-ADepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS B2rescue A70D
Plasmid#78658PurposeGenerates B2 inserts suitable for rescueDepositorInsertB2rescue cassette
ExpressionMammalianAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAT.011
Plasmid#119555PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.167
Plasmid#119636PurposeLevel 0 Part. Insertion siteDepositorInsert6803 NS2 Up Flank
UseSynthetic BiologyMutationnon functional/large non-specific insertionAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.168
Plasmid#119635PurposeLevel 0 Part. Insertion siteDepositorInsert6803 NS2 Down Flank
UseSynthetic BiologyMutationbp 630 A to TAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_EYFP
Plasmid#167858PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_117/pcDNA3
Plasmid#51985Purposemammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nMDepositorInsertNano-lantern_CaM-2GS
ExpressionMammalianMutationS257G in Rluc8Available SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only