We narrowed to 9,800 results for: otos

1. pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

   Plasmid

   #216276

   Purpose

   AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   NES-jRGECO1a
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   human synapsin 1 (hSyn)

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W

   Plasmid

   #208552

   Purpose

   Lentiviral vector expressing gRNA targeting human RUNX3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   RUNX3(13) (RUNX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W

   Plasmid

   #208551

   Purpose

   Lentiviral vector expressing gRNA targeting human RUNX3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   RUNX3(12) (RUNX3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W

   Plasmid

   #210612

   Purpose

   Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   Control(1)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W

   Plasmid

   #200491

   Purpose

   Lentiviral vector expressing gRNA targeting human RIC8A

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   RIC8A(16) (RIC8A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W

   Plasmid

   #200490

   Purpose

   Lentiviral vector expressing gRNA targeting human RIC8A

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   RIC8A(15) (RIC8A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

   Plasmid

   #210613

   Purpose

   Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   Control(2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Qt-FLAG-TGA-rt9us-antiGFP

   Plasmid

   #206954

   Purpose

   Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   QtEnc, antiGFP-intrabody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psicheck2-PHLPP2mut

   Plasmid

   #203606

   Purpose

   Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

   Depositor

   Hiroyuki Mizuguchi

   Article

   Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

   Insert

   PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   miR-27b target sequences in the 3'UTR were m…

   Promoter

   SV40

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CSII-TK-B4GALT3

    Plasmid

    #203598

    Purpose

    Lentiviral vector to express B4GALT3 in mammalian cells

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    beta-1,4-galactosyltransferase 3 (B4GALT3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TK

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CSII-TK-MAIP1

    Plasmid

    #203599

    Purpose

    Lentiviral vector to express MAIP1 in mammalian cells

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    matrix AAA peptidase interacting protein 1 (MAIP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TK

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psicheck2-B4GALT3

    Plasmid

    #203601

    Purpose

    Reporter plasmid carrying B4GALT3 3'UTR

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    beta-1,4-galactosyltransferase 3 (B4GALT3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    nt 1800-2330 3'UTR

    Promoter

    SV40

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. psicheck2-MAIP1

    Plasmid

    #203603

    Purpose

    Reporter plasmid carrying MAIP1 3'UTR

    Depositor

    Hiroyuki Mizuguchi

    Article

    Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

    Insert

    matrix AAA peptidase interacting protein 1 (MAIP1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Qt-FLAG-TAA-rt20s-antiGFP

    Plasmid

    #206955

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMRX-IRES-puro-3xFLAG

    Plasmid

    #204545

    Purpose

    Expresses 3xFLAG tag in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    3xFlag
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNMSB96

    Plasmid

    #199316

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P-1-MVP

    Plasmid

    #204541

    Purpose

    Expresses GST-tagged MVP in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    MVP (MVP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. H6GB1tevP53(304-393)

    Plasmid

    #200313

    Purpose

    bacterial expression of p53 TET-CTD

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53(304-393) (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. H6GB1tevP53(363-393)

    Plasmid

    #200314

    Purpose

    bacterial expression of P53 CTD

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53(363-393) (TP53 Human)
    

    Use

    Tags

    His6-GB1-tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCas-mut4/1_2_VB

    Plasmid

    #186444

    Purpose

    Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas4 domain (K81A)

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV/EF1a::DIO-iLMO2

    Plasmid

    #129482

    Purpose

    AAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

    Depositor

    Robert Gross

    Article

    Jaiswal et al J Neurosci Res. 2017 Aug 3. doi: 10.1002

    Insert

    iLMO2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. P2061

    Plasmid

    #186299

    Purpose

    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

    Inserts

    LexA-HBD-B112
    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    2xLexop-minimalCyc1 and PACT1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEE005

    Plasmid

    #176762

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    101
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV(MinDis)-iEscSnFR_PM

    Plasmid

    #182813

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_PM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV(MinDis)-iEscSnFR_cyto

    Plasmid

    #182814

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_cyto
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV(MinDis)-iFluoxSnFR_ER

    Plasmid

    #182815

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iFluoxSnFR_ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV(MinDis)-iFluoxSnFR_PM

    Plasmid

    #182816

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iFluoxSnFR_PM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV(MinDis)-iFluoxSnFR_cyto

    Plasmid

    #182817

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iFluoxSnFR_cyto
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV9-hSyn-iEscSnFR_ER

    Plasmid

    #182818

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_ER
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV9-hSyn-iEscSnFR_PM

    Plasmid

    #182819

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

    Insert

    iEscSnFR_PM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge