We narrowed to 22,109 results for: ESC

1. pEB2-DsRed-Express

   Plasmid

   #103995

   Purpose

   Plasmid encoding DsRed-Express

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   DsRed-Express
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBAD-KIRIN1-GR

   Plasmid

   #107417

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1-GR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCH1 - pBait-empty

   Plasmid

   #174663

   Purpose

   arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-D1cpV

   Plasmid

   #37479

   Depositor

   Amy Palmer, Roger Tsien

   Article

   Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

   Insert

   D1cpV second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pEB1-mCerulean3

   Plasmid

   #103973

   Purpose

   Plasmid encoding mCerulean3

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mCerulean3
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, Y66W, …

   Promoter

   proC

   Available since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFBNb-cALFA

   Plasmid

   #159990

   Purpose

   For the protein expression with ALFA tag at C-terminus in insect cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mRuby2-Lifeact-7

   Plasmid

   #54674

   Purpose

   Localization: Actin, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   LifeAct-mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. mEos2-H2B-6

   Plasmid

   #57384

   Purpose

   Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pBSV2G_PflaB-msfYFPBb

   Plasmid

   #118233

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven msfYFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-shRNA-Tet1

    Plasmid

    #85742

    Purpose

    shRNA against mouse Tet1

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA Tet1
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMito-iRFP720

    Plasmid

    #45464

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-GR-AKAR3

    Plasmid

    #173015

    Purpose

    Improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    GR-AKAR3
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. VV020: WT Arch-3 - eGFP in pET28b

    Plasmid

    #58488

    Purpose

    Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3
    

    Use

    Tags

    eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CycA-Venus-Flag (1305)

    Plasmid

    #39853

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Insert

    Cyclin A (CCNA2 Human)
    

    Use

    Tags

    Flag and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

15. mCerulean3-Lysosomes-20

    Plasmid

    #55428

    Purpose

    Localization: Lysosome Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    LAMP1
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTCW012

    Plasmid

    #83527

    Purpose

    Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ii-Str_SBP-mCherry-GPI

    Plasmid

    #65297

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PET-3a-chpBK

    Plasmid

    #155201

    Purpose

    To express recombinant ChpBK protein

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Inserts

    chpS-chpBK-6×His-Tag
    GyrA-CBD-10xHis-Tag
    

    Use

    Tags

    10xHis and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYL322d1/eGFP-C

    Plasmid

    #183152

    Purpose

    Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-mRhubarb713

    Plasmid

    #197207

    Purpose

    Expresses the protein of mRhubarb713 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mRhubarb713
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSW002

    Plasmid

    #111248

    Purpose

    Broad host-range bacterial vector

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBSV2G_PflaB-mCeruleanBb

    Plasmid

    #118235

    Purpose

    Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCerulean

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    flagellin promoter driven mCerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. myr-palm_2xmEYFP

    Plasmid

    #127811

    Purpose

    Homo-dimer mEYFP-mEYFP expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    myristoylated-palmitoylated-mEYFP-mEYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Aurein1.2_(+36GFP)-Cre

    Plasmid

    #71748

    Purpose

    Aurein1.2+36GFP-Cre fusion expression in E. Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_Aurein1.2+36GFP-Cre
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSW002-Pc

    Plasmid

    #108234

    Purpose

    Broad host bacterial expression vector with constitutive Pc promoter.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    Pc promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFA6a-link-yoTagRFP657-SpHis5

    Plasmid

    #44846

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNIR_PKAbiosensor

    Plasmid

    #127439

    Purpose

    Mammalian expression of the NIR biosensor for detecting PKA activity

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    NIR PKA biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSYC-97

    Plasmid

    #31565

    Depositor

    Seok-Yong Choi

    Article

    Kim et al PLoS One. 2011 . 6(4):e18556.

    Insert

    pCS4+-NLS-EGFP-P2A-mCherry-CAAX
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIREShygro HA-mVenus-mAID

    Plasmid

    #117714

    Purpose

    Expression of HA-tagged mVenus fused to mAID

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mVenus-mAID
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNIR_JNKbiosensor

    Plasmid

    #127440

    Purpose

    Mammalian expression of the NIR biosensor for detecting JNK activity

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    NIR JNK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKDR-Lenti-TetOn

    Plasmid

    #183555

    Purpose

    pKDR compatible TetOn lentiviral transfer plasmid

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Launchpad (AVA2590)

    Plasmid

    #85442

    Purpose

    Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    FRT-flanked EF1alfa-GFP PGK-Puromycin cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

33. mTagRFP-T-Cx43-7

    Plasmid

    #58009

    Purpose

    Localization: Gap Junctions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Cx43
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pNOC-stacked-aequorin

    Plasmid

    #101010

    Purpose

    Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis

    Tags

    Expression

    Mutation

    Promoter

    Ribi

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMYO127TAG-SepT

    Plasmid

    #34625

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin-127TAG
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized + an amber stop codon was introdu…

    Promoter

    Ipp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-N1-ro2GFP

    Plasmid

    #82370

    Purpose

    Expression of redox sensitive GFP in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. VAMP2-SNAPtag

    Plasmid

    #107372

    Purpose

    Synaptic vesicle protein VAMP2 fused to the SNAPtag domain

    Depositor

    David Perrais

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    SNAPtag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEB2-Katushka

    Plasmid

    #104008

    Purpose

    Plasmid encoding Katushka

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Katushka
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PEP101E

    Plasmid

    #97387

    Purpose

    binary construct of cytosolic sfGFP1-10 for plant

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBAD-6xHis-BglII

    Plasmid

    #196654

    Purpose

    Derived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII site

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Type

    Empty backbone

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge