We narrowed to 18,888 results for: REV

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UKNP1.2.4

Plasmid

#97359

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.2.5

Plasmid

#97360

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-5/5

Plasmid

#91464

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-5/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-5/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-1/5

Plasmid

#91351

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-1/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-3/5

Plasmid

#91352

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-3/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (D1320A)

Plasmid

#41586

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

D1320A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
TIAM1_PDZ_1

Plasmid

#103925

Purpose

Protein expression and purification of TIAM PDZ domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TIAM1 (TIAM1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPMVAugw

Plasmid

#98300

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TEFM1EfmvaE>TEBS1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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DsRed2_pLENTI-CAG-IRES-GFP

Plasmid

#177010

Purpose

Mammalian lentiviral expression vector encoding DsRed2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B2-GFP

Plasmid

#123109

Purpose

Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pQE-nTR

Plasmid

#21812

Depositor

Wafik El-Deiry

Article

Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

Insert

pQE-nTR (TNFSF10 Human)

Expression

Bacterial

Mutation

His tag and aa95-113 were removed from pQE-hTR

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pHH0103_SNX9-1/1

Plasmid

#91513

Purpose

Protein expression and purification of human SH3 domain construct SNX9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX9-1/1 (SNX9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-137

Plasmid

#26327

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-137 (Mir137 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-375

Plasmid

#26336

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-375 (Mir375 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pDendra2-Hygro

Plasmid

#202407

Purpose

Dendra2 expression plasmid

Depositor

Morito Kurata

Article

Sugita et al Commun Biol. 2023 Jun 1;6(1):582. doi: 1

Insert

Hygromycin

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2803

Plasmid

#63646

Purpose

pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p-715MRPprom-MRPcucuc

Plasmid

#87199

Purpose

Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

RMRP (RMRP Human)

Expression

Mammalian

Mutation

GAGAG91-95 to CUCUC

Promoter

human RMRP

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO15A-1/2

Plasmid

#91491

Purpose

Protein expression and purification of human SH3 domain construct MYO15A-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO15A-1/2 (MYO15A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LINXb3

Plasmid

#73615

Purpose

Light-induced nuclear export switch for microscopy in mammalian cells.

Depositor

Brian Kuhlman

Article

Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

Insert

LINXb3

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2899(X-2 MarkerFree)

Plasmid

#73271

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

Plasmid

#63170

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-OLLAS"

Tags

N-myristoylation

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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UKNP4.3.2

Plasmid

#98270

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
UKNP3.2.3

Plasmid

#98248

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
UKNP3.1.1

Plasmid

#98214

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLexm-gfp-Pept2

Plasmid

#167988

Purpose

expresses rat PepT2 with a c terminal cleavable GFP-His tag.

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

PepT2 (Slc15a2 Rat)

Tags

cleavable GFP-his8

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV2-2/2

Plasmid

#91506

Purpose

Protein expression and purification of human SH3 domain construct VAV2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV2-2/2 (VAV2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
AAV-CAG-FLEX-NEUROD1-T2A-mCherry

Plasmid

#178584

Purpose

Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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UKNP4.2.1

Plasmid

#98267

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pV1464

Plasmid

#111440

Purpose

N. castellii Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
UKNP2.3.1

Plasmid

#98210

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV114

Plasmid

#63181

Purpose

pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17

Plasmid

#121386

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Promoter

ADH1 promoter

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 219-465

Plasmid

#45972

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 219-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEK20

Plasmid

#210582

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdGAPDHpro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdGAPDHpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CMV-CIBN-mCherry-VAMP2

Plasmid

#178573

Purpose

Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CIBN-mCherry-VAMP2

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mcherry Beta 4

Plasmid

#36114

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B4 (Gnb4 Mouse)

Tags

mcherry

Expression

Mammalian

Promoter

cmv

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pCFL-mPML-3KRmut-iB

Plasmid

#140287

Purpose

Expression of mouse PML KR mutant

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

PML (Pml Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgMPC2_7

Plasmid

#163457

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 7 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgMPC2_9

Plasmid

#163458

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (MPC2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mcherry Beta 3

Plasmid

#36113

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B3 (GNB3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

cmv

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
px330-MRPsg1

Plasmid

#87189

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 1

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #1 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCETN1-IRFP670

Plasmid

#99879

Purpose

Expression of CETN1-iRFP670 in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

CETN1 (CETN1 Human)

Tags

iRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415-Cas9-VPR

Plasmid

#163971

Purpose

Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

Depositor

Jiazhang Lian

Article

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Insert

Cas9-VPR

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB2804

Plasmid

#63644

Purpose

pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SORBS3-1/3

Plasmid

#91534

Purpose

Protein expression and purification of human SH3 domain construct SORBS3-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS3-1/3 (SORBS3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_VAV3-1/2

Plasmid

#91528

Purpose

Protein expression and purification of human SH3 domain construct VAV3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV3-1/2 (VAV3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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