We narrowed to 4,880 results for: pir

1. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

   Plasmid

   #140193

   Purpose

   Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   dLbCas12a-VPR
   Bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   

   Use

   CRISPR and Synthetic Biology; Fungal genome integ…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNAse deactivated

   Promoter

   PgpdA and PtrpC

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pαH-S-GSAS-B.1.351.v2

   Plasmid

   #173786

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPHAGE-CR-NENF-C-TAP

   Plasmid

   #128511

   Purpose

   Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   NENF (NENF Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Synonymous silent mutations to F81, Y82, G83 and …

   Promoter

   CMV

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pαH-S-GSAS-B.1.351.v6

   Plasmid

   #173791

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein South African variant version 6

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLD-puro-Cc-PARK7WT-VA

   Plasmid

   #115182

   Purpose

   Lentiviral transduction and expression of PARK7WT into any mammalian cell

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7WT (PARK7 Human)
   

   Use

   Lentiviral

   Tags

   Versatile affinity tag (2XStreptactin II-6XHis-3X…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTM-RBDv2

   Plasmid

   #162785

   Purpose

   SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells

   Depositor

   Ivan Vorobiev

   Article

   Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

   Insert

   SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
   

   Use

   Tags

   10xHis, c-myc, and hTPA leader

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pαH-S-GSAS-B.1.351.v3

   Plasmid

   #173787

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein South African variant version 3

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pαH-S-GSAS-B.1.351

   Plasmid

   #171749

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pαH-S-GSAS-D614G-K417N-E484K-N501Y

   Plasmid

   #171750

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-K417N-E484K-N501Y

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-D614G-K417N-E484K-N501Y variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pαH-S-GSAS-D614G-deltaFV

    Plasmid

    #171743

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein mink variant (S-GSAS-D614G-deltaFV variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-D614G-deltaFV variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPHAGE-C20orf24- C20orf24-3’UTR-WT

    Plasmid

    #128508

    Purpose

    Lentiviral constitutive expression of C20orf24 under control of its native WT 3'UTR of human C20orf24.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    RAB5IF (RAB5IF Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis-TEV-Nsp15-E266Q

    Plasmid

    #218803

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    E266Q (G to C)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis-TEV-Nsp15-V66L

    Plasmid

    #219524

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    V66L (G to C)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHis-TEV-Nsp15-A81V

    Plasmid

    #219525

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    A81V (C to T)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHis-TEV-Nsp15-D183N

    Plasmid

    #219526

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    D183N (G to A)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHis-TEV-Nsp15-H234A

    Plasmid

    #219529

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    H234A (CAC to GCG)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTwist-SARS-CoV-2 Spike-deltaC18 deltaE156-F157+R158G (GGA)

    Plasmid

    #212421

    Purpose

    Encodes SARS-CoV-2 spike E156-F157deletion,R158G for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike E156-F157deletion,R158G (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E156-F157deletion,R158G

    Promoter

    CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSF_his6_sumo_hRNMT_RAM

    Plasmid

    #213528

    Purpose

    Expresses the human RNMT-RAM complex

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Inserts

    human RNMT (RNMT Human)
    RAM
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF_his6_sumo_SARS_CoV2_NSP14

    Plasmid

    #213518

    Purpose

    Expresses NSP14 of SARS-CoV-2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    N-terminal His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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