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We narrowed to 462 results for: Pef1

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Showing: 51 - 100 of 462 results

  12. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Article
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available Since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100547-plasmid-map-sequence-id-313417

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  13. pEF1-Pls3-sfGFP

    Plasmid
    #205750
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls3 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205750-plasmid-map-sequence-id-478438

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  18. pEF1-Pls1-sfGFP

    Plasmid
    #205748
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls1 (Phyperspank mutant, IPTG inducible)
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205748-plasmid-map-sequence-id-483889

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  26. pEF1-Pls4-sfGFP

    Plasmid
    #205751
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls4 (Phyperspank mutant, IPTG inducible)
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    205751-plasmid-map-sequence-id-478437

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  30. pEF1alpha-mClover3-bGHpA

    Plasmid
    #172286
    Purpose
    Constitutive expression of mClover3
    Depositor
    Patrik Verstreken
    Insert
    mClover3
    Expression
    Bacterial
    Promoter
    EF1alpha
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172286-plasmid-map-sequence-id-340305

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  31. pEF1-His-NEDD8

    Plasmid
    #159322
    Purpose
    Mammalian cell expression of mouse His-NEDD8
    Depositor
    Jianjun Zhao
    Insert
    His-NEDD8 (Nedd8 Mouse)
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159322-plasmid-map-sequence-id-311864

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  34. pEF1-MH.Bl-mDcrSOM

    Plasmid
    #120540
    Purpose
    protein expression
    Depositor
    Petr Svoboda
    Insert
    Dicer (Dicer1 Mouse)
    Expression
    Mammalian
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    120540-plasmid-map-sequence-id-240211

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  35. pEF1-MH.Bl-mDcrOO

    Plasmid
    #120541
    Purpose
    protein expression
    Depositor
    Petr Svoboda
    Insert
    Dicer (Dicer1 Mouse)
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    120541-plasmid-map-sequence-id-244344

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  36. pEF1alpha-rTetR-bGHpA

    Plasmid
    #172285
    Purpose
    Constitutive expression of reverse tetracycline-controlled transactivator
    Depositor
    Patrik Verstreken
    Insert
    rTetR
    Expression
    Bacterial
    Promoter
    EF1alpha
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172285-plasmid-map-sequence-id-340294

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  38. pEF1-puro/hpb1

    Plasmid
    #59976
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 headpiece (ITGB1 Human)
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Promoter
    EF1a promoter
    Available Since
    Sept. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. pEF1-Pls2-sfGFP

    Plasmid
    #205749
    Purpose
    Expresses sfGFP under IPTG inducible promoter Pls2 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/low strength.
    Depositor
    Jerome Bonnet
    Article
    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95
    Type
    Empty backbone
    Use
    Shuttle vector gram+ gram-
    Expression
    Bacterial
    Promoter
    Pls2 (Phyperspank mutant, IPTG inducible)
    Available Since
    Aug. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    205749-plasmid-map-sequence-id-483890

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  48. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  50. pEF1α-M2rtTA-T2A-Puro

    Plasmid
    #169175
    Purpose
    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174
    Depositor
    Igor Slukvin
    Article
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    M2rtTA-T2A-Puro
    Use
    Piggybac transposon
    Expression
    Mammalian
    Mutation
    See Depositor Comments Below
    Promoter
    EF1a
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169175-plasmid-map-sequence-id-362474

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Showing: 51 - 100 of 462 results