We narrowed to 30,855 results for: puro

1. Puro-iNotch1IC

   Plasmid

   #75338

   Purpose

   doxycycline inducible Notch1 intracellular domain gene over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   Notch1 Intracellular domain (Notch1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiGuide_CjCas9-Puro

   Plasmid

   #202564

   Purpose

   hU6-BsmBI-gRNA-Puro

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   CjCas9 sgRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HP138-puro

   Plasmid

   #134246

   Purpose

   Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   Contains Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVKG1-Puro

   Plasmid

   #108670

   Purpose

   Puromycin resistant cassette for knock-in by VIKING method

   Depositor

   Shigeo Sugano

   Article

   Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

   Insert

   PURO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB1610_pRR-Puro

   Plasmid

   #65853

   Purpose

   Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

   Depositor

   Marc Bühler

   Article

   Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

   Insert

   split puromycin N-acetyltransferase coding sequence
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. SaCas9v3-Puro

   Plasmid

   #178813

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiUniversal-Puro

   Plasmid

   #127749

   Purpose

   Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Puro-iDEST

   Plasmid

   #75336

   Purpose

   GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   TRE-DEST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTopo_Ex2Donor_pPGK-PURO

    Plasmid

    #198862

    Purpose

    Donor template for CRISPR/Cas9 targeting of TERT Ex2

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    PURO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Puro 2.0

    Plasmid

    #24970

    Depositor

    Tyler Jacks

    Article

    Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGCGFP-puro

    Plasmid

    #31266

    Depositor

    Andrew Pierce

    Insert

    GCGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiGuideFB-Puro-A

    Plasmid

    #192506

    Purpose

    For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSc1-puro

    Plasmid

    #80438

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell, and also confers resistance to puromycin

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    Puromycin resistance
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6, and hPGK

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINS-Puro

    Plasmid

    #31389

    Depositor

    Yegor Vassetzky

    Article

    Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

    Insert

    pac
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBLIC-puro

    Plasmid

    #45197

    Purpose

    a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.

    Depositor

    Priyamvada Rai

    Article

    Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pXRDRE-puro

    Plasmid

    #31270

    Depositor

    Andrew Pierce

    Insert

    XRDRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgVRK2-puro

    Plasmid

    #199636

    Purpose

    sgRNA guide against VRK2

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgLacZ-puro

    Plasmid

    #199635

    Purpose

    sgRNA control guide targeting bacterial LacZ

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgCTRL-puro

    Plasmid

    #199634

    Purpose

    sgRNA control guide targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge