We narrowed to 8,514 results for: sgrna

1. pKDsgRNA-hisG

   Plasmid

   #89954

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   hisG gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-U6-AasgRNA3.8.3

   Plasmid

   #121959

   Purpose

   Mammalian expression, Transcription regulation, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b single chimeric gRNA, MS2 hairpin inserted
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-sgRNA_Drosha_1

   Plasmid

   #79860

   Purpose

   CRISPR/Cas9 DROSHA

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   sgRNA Drosha
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pX330-sgRNA_Drosha_2

   Plasmid

   #79861

   Purpose

   CRISPR/Cas9 DROSHA

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   sgRNA Drosha
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pX330-sgRNA_Drosha_3

   Plasmid

   #79862

   Purpose

   CRISPR/Cas9 DROSHA

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   sgRNA Drosha
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pX330-sgRNA_Dicer_1

   Plasmid

   #68807

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MYC sgRNA4

   Plasmid

   #100556

   Purpose

   Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   MYC sgRNA4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEX-A-U6-MiSgRNA_PuroR

   Plasmid

   #84781

   Purpose

   Expresses minor satellite-specific sgRNA.

   Depositor

   Heinrich Leonhardt

   Article

   Schmidtmann et al Nucleus. 2016 Sep 27:0.

   Insert

   minor satellite-specific sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDsgRNA-trmI

   Plasmid

   #89955

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   trmI gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDsgRNA-pfkB

    Plasmid

    #102284

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    pfkB gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKDsgRNA-xylA

    Plasmid

    #89964

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    xylA gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKDsgRNA-lpoB

    Plasmid

    #89959

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    lpoB gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-sgRNA_Dgcr8_3

    Plasmid

    #73527

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pX330-sgRNA_Dgcr8_4

    Plasmid

    #73528

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pX330-sgRNA_Dgcr8_2

    Plasmid

    #73526

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pSEVA63_sgRNA-B0_M13ps

    Plasmid

    #231421

    Purpose

    M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEVA63_sgRNA-B1_M13ps

    Plasmid

    #231422

    Purpose

    M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJC.15A.sgRNA.TA

    Plasmid

    #232483

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Inserts

    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3 constitutive promoter and native promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX330-sgRNA006_CTCF

    Plasmid

    #232928

    Purpose

    Expression vector for a sgRNA against the mouse CTCF locus and SpCas9.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9 (Ctcf S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX459-sgRNA047_Podxl

    Plasmid

    #232932

    Purpose

    Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Podxl S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge