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pcDNA5-hygro-eGFP-HEC1

Plasmid

#114027

Purpose

Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC3

Plasmid

#183299

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMBP-SpyCatcher

Plasmid

#79053

Purpose

Expresses maltose binding protein (MBP) SpyCatcher fusion

Depositor

James Bowie

Article

Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

Insert

MBP-SpyCatcher

Tags

6xHis and Maltose binding protein

Expression

Bacterial

Mutation

Derived from pDEST14-SpyCatcher (Plasmid #35044)…

Promoter

T7

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT1

Plasmid

#183282

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT1

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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tdTomato-LacI

Plasmid

#183921

Purpose

Lac repressor labeled with tdTomato; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdTomato-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCI118-SiT-Cas12a-[Ind]

Plasmid

#128406

Purpose

Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_2pabPAC(Y72L)

Plasmid

#158970

Purpose

A negative control for AAV-mediated 2P-activatable bPAC

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPACY72L

Use

AAV

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

Plasmid

#134857

Purpose

Expresses EGFP-tagged synaptic vesicle marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 3C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PDGFRB

Plasmid

#183314

Purpose

All-in-One CRISPRko system with a guide RNA that targets PDGFRB gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PDGFRB

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Positron-ST

Plasmid

#129267

Purpose

Cre-dependant expression of soma-localized Positron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

AAV

Expression

Mammalian

Mutation

D92N, E199V

Promoter

hsyn

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

SEAP

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLCRISPR-CMV

Plasmid

#102609

Purpose

Control lentivector expressing Cas9 and gRNA scaffold

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huErCas12a

Plasmid

#174685

Purpose

Expresses 6xHis-MBP tagged ErCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huErCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis

Expression

Bacterial

Mutation

Q926R mutation is observed as indicated from the …

Promoter

T7

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Zeo

Plasmid

#227261

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPCK

Plasmid

#133128

Purpose

a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

PEP-carboxykinase gene

Expression

Bacterial

Promoter

PEP-carboxykinase promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_h7SK

Plasmid

#102842

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_h7SKpromoter

Use

As pcr template

Promoter

h7SK

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-KG-WASP-GBD

Plasmid

#30113

Depositor

David Sacks

Article

Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-

Insert

WASP-GBD (WAS, WAS Human)

Tags

GST

Expression

Bacterial

Mutation

Contains the Cdc42-interacting domain of WASP (aa…

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pAC1430

Plasmid

#127981

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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